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Home > Medicine & Health Science textbooks > Pre-clinical medicine: basic sciences > Anatomy > Effects of Activated Microglia on the Properties of Neural Stem Cells in Vitro
Effects of Activated Microglia on the Properties of Neural Stem Cells in Vitro

Effects of Activated Microglia on the Properties of Neural Stem Cells in Vitro


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About the Book

This dissertation, "Effects of Activated Microglia on the Properties of Neural Stem Cells in Vitro" by Xuqing, Liu, 刘绪卿, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Neural stem cell (NSC) transplantation strategy offers great potential to treat spinal cord injury (SCI). NSCs may replace lost neurons or oligodendrocytes, and act as a source of neurotrophic factors to support the survival of remaining cells. Their efficiency was limited by poor survival after transplantation, and they had more tendencies to differentiate into astrocytes, but not neurons and oligodendrocytes. This project investigated whether activated microglia is a factor that contributes to this phenomenon, and studied the potential role of minocycline, a widely used antibiotic drug, to modify the negative effects of microglia on NSCs. In the first part of this study, we used organotypic spinal cord slice (SCS) culture to mimic in vivo local environment after SCI, and NSCs were grafted on their surface or shared culture medium with them. After specific depletion of microglia with clodronate loaded liposome, more grafted NSCs survived, and in the co-culture system, the NSC neuronal differentiation rate increased while glial differentiation rate decreased, the apoptosis rate also decreased. This suggested that activated microglia may impair NSC survival, and neuronal differentiation, but improve glial differentiation. In the second part of this study, we first tested the direct effects of minocycline on NSC apoptosis, proliferation and differentiation in vitro, to test whether minocycline has any side effect on NSCs. The results showed that at the concentration 10μg/ml or lower, minocycline did not affect NSC survival and proliferation, but impaired neuronal differentiation. Then we treated primary microglia culture with LPS or LPS plus minocycline, and collected the conditioned mediums (CM-LPS and CM-LPSMC) to test their effects on NSC apoptosis and differentiation. The results showed that compared with CM-LPS, CM-LPSMC resulted in a significantly lower apoptotic rate of NSCs, also allowed NSC neuronal differentiation. This suggested that minocycline may impair the pro-apoptotic effect of activated microglia on NSCs. In conclusion, our study showed that activated microglia may impair NSC survival and neuronal differentiation. This indicated that in NSC transplantation strategy for SCI, microglia would be a target to be manipulated to improve graft survival and neuronal differentiation. Although minocycline may suppress NSC differentiation towards neurons, it has the potential to protect NSCs from the toxic effects of activated microglia. This showed the therapeutic potential of minocycline in NSC transplantation strategies for SCI. DOI: 10.5353/th_b4723600 Subjects: Microglia Neural stem cells


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Product Details
  • ISBN-13: 9781361303955
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 156
  • Weight: 376 gr
  • ISBN-10: 1361303956
  • Publisher Date: 26 Jan 2017
  • Binding: Paperback
  • Language: English
  • Spine Width: 8 mm
  • Width: 216 mm


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