Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (Rsps) as a Model
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Home > Medicine & Health Science textbooks > Medical specialties, branches of medicine > Pathology > Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (Rsps) as a Model
Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (Rsps) as a Model

Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (Rsps) as a Model


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About the Book

This dissertation, "Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (RSPs) as a Model" by Chung-yan, Joanne, Lo, 羅頌恩, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Dengue is the most common mosquito-borne human disease, leading to 2.5 billion people at risk, 50-100 millions infections each year worldwide and among them, 500 000 severe dengue cases (dengue hemorrhagic fever, DHF/ dengue shock syndrome, DSS) plus more than 20 000 deaths. It can be caused by any of four dengue virus serotypes, which are antigenicly distinct and belong to the Flaviviridae family, genus Flavivirus. However, up till now there is no specific drug and vaccine against dengue. Understanding mechanisms developed by dengue virus to exploit host cells during all stages of the replication cycle is a first step towards the rationale design of anti-viral strategies. Very little is known about the late stages, which consist of assembly, budding and secretion of the virus. It is therefore very important to develop tools in order to study the egress of the virus. In this study, I investigated a stable cell line named Hela-prME that expresses serotype 1 dengue virus (DENV-1) prM and E native structural envelope proteins and constitutively produces dengue recombinant subviral particles (RSPs). Biochemical characterization of DENV-1 RSPs has validated that this cell line is a potential tool to study the dengue viral late-stage. Indeed, the maturation process observed with RSPs is similar to the pathway described for real virus (cleavage of prM fragment, homodimerization of E, acquisition of complex sugars). To better understand and depict the dengue virus late-stage secretion, I combined various electron microscopy (EM) techniques e.g. classical transmission electron microscopy (TEM), negative staining, immunogold labeling on cryo-ultrathin sections (Tokuyashu method) and tomography (ET) with such RSPs tool. The EM results obtained illustrate that electron dense particles and tubules labeled by antibodies directed against E and prM proteins were abundantly found in the lumen of endoplasmic reticulum (ER)-related cisternae of HeLa prME cells. Epositive particles were also found in other structures such as Golgi stacks and vesicles nearby as well as in aggregates with electron dense materials inside and surrounded by membrane. These particles are most likely corresponding to DENV-1 RSPs whereas the tubules may be other structures induced by assembly of prM and E proteins. This study has clearly shown that DENV-1 RSPs assemble in the ER and transport through the secretory pathway before being released. This work further validates the use of dengue RSPs and RSPs-producing cells as a model to study viral egress. DOI: 10.5353/th_b4833011 Subjects: Electron microscopes Dengue viruses


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Product Details
  • ISBN-13: 9781361281192
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 118
  • Weight: 567 gr
  • ISBN-10: 1361281197
  • Publisher Date: 26 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 8 mm
  • Width: 216 mm


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Characterization by Electron Microscopy of Dengue Virus Egress Using Dengue Recombinant Subviral Particle (Rsps) as a Model
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