Buy New Insights Into Microbial Metal Resistance by Omics-Based Approaches
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Science, Technology & Agriculture > Civil engineering, surveying and building > New Insights Into Microbial Metal Resistance by Omics-Based Approaches
New Insights Into Microbial Metal Resistance by Omics-Based Approaches

New Insights Into Microbial Metal Resistance by Omics-Based Approaches


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This dissertation, "New Insights Into Microbial Metal Resistance by Omics-based Approaches" by Liguan, Li, 李麗觀, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Aiming to gain a thorough understanding of microbial metal resistance under the current state of metal contamination, with the omics methods, metal resistance genes (MRGs) were explored from different perspectives in this thesis within a framework of environmental relevance. To give a glimpse of genetic characteristics associated with "metallophiles" - Cupriavidus metallidurans, extensive comparison among genomes (including 4 isolates) from the corresponding genus was conducted, revealing the most diverse and compact MRG clusters owned by the species. Moreover, copper enrichment was obtained from activated sludge and analyzed by metagenomics. In addition to particular perturbation of copper stress on community composition, induced shift of resistance genes abundance toward copper efflux system was observed based upon efficient analysis pipeline coupled with the self-constructed copper resistance protein database (CuRD). Given the resolution of a vast number of genomes owning to the recent advent of de novo sequencing platforms, broad-scale MRGs profile and their association with antibiotic resistance genes (ARGs) were investigated through efficient bioinformatic analysis. Significant MRG enrichment was observed in cosmopolitan taxa (e.g. Proteobacteria and Enterobacteriales) and human habitat. Regarding the relationship between MRGs and ARGs in the large genome collection, strong abundance correlation in several phylogenies (e.g. Proteobacteria, Gamaproteobacteria, and Enterobacteriales) and significant genetic co-occurrence in human pathogen species (e.g. Escherichia coli, Salmonella enterica, Shigella flexneri) were discovered. Furthermore, abundance/diversity profiles and similarity patterns of MRGs in 57 metagenomic datasets from 10 typical human-related environmental types were studied. An obvious abundance range was found in different environmental types and specific enrichment was revealed in adult chicken faeces. Distinct environmental types acted as a primary role in shaping similarity grouping in NMDS of the 57 samples. Though the main searching tool utilized in the thesis is similarity-based BLAST, it highly depends on reference sequences thereby restricting prediction of novel genes. Therefore, domain-based profile hidden markov models (HMMs) database - MR.HMMs composed of 60 metal resistance protein families was constructed in this study. By examining precision and recall values, profile specific GA threshold was set as a reliable cutoff rather than e-value. To further optimize performance, public database UniPro/SwissProt was utilized as an independent training set to guarantee excellent retrieval capability for target MRGs. By applying the MR.HMMs-based searching strategy to metagenomic datasets of livestock faeces, higher MRGs annotation percentages were revealed compared with similarity-based BLAST searching. At last, as a preliminary experiment to explore multi-omics application in microbial metal resistance, responses at gene transcription and translation levels in the model strain Escherichia coli K12 were assessed by high-throughput sequencing (HTS)-based metatranscriptome and mass spectrum based metaproteome. The inconsistence between RNA and protein implied remarkable complexity across different biological processes, which substantially limited parallel analysis of results obtained from the two platforms. Differential regulation analysis revealed that toxic effect of silver w


Best Sellers


Product Details
  • ISBN-13: 9781361013786
  • Publisher: Open Dissertation Press
  • Publisher Imprint: Open Dissertation Press
  • Height: 279 mm
  • No of Pages: 206
  • Weight: 767 gr
  • ISBN-10: 1361013788
  • Publisher Date: 26 Jan 2017
  • Binding: Hardback
  • Language: English
  • Spine Width: 13 mm
  • Width: 216 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
New Insights Into Microbial Metal Resistance by Omics-Based Approaches
Open Dissertation Press -
New Insights Into Microbial Metal Resistance by Omics-Based Approaches
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

New Insights Into Microbial Metal Resistance by Omics-Based Approaches

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!