Laboratory Manual for Microbiology Fundamentals: A Clinical Approach
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Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

Laboratory Manual for Microbiology Fundamentals: A Clinical Approach


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About the Book

Laboratory Manual for Microbiology Fundamentals: A Clinical Approach is a compilation of decades of experience between the two authors, Steven Obenauf and Susan Finazzo. Their approach will add to your own cadre of techniques for effective teaching, and help your students avoid many of the common pitfalls encountered in the lab. This lab is very visually informative, and has a conversational approach which will entice student interest and facilitate learning and success in your course.

Table of Contents:
Laboratory Overview Introduction Laboratory Safety, Equipment, and Materials Tools of the Laboratory: Basic Techniques 1 Use of the Microscope 2 Aseptic Techniques 3 Isolation Streak Plate Eukaryotic Cells and Microorganisms 4 Microbial Phototrophs: Algae and Cyanobacteria 5 Protozoa 6 Fungi 7 Bacterial Motility: Hanging Drop Method Methods for Microscopic Analysis: Staining 8 Simple Staining and Smear Preparation 9 Negative Stain 10 Capsule Stain 11 Gram Stain 12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods) 13 Endospore Stain (Schaeffer-Fulton Method) Methods for Culturing and Diagnosing Microbes: Media 14 Blood Agar 15 Mannitol Salt Agar 16 Eosin Methylene Blue Agar 17 MacConkey Agar Microbial Nutrition, Ecology, and Growth 18 Nutrients and Growth: Chemical Content of Media 19 Osmotic Pressure and Growth 20 Oxygen and Growth: Aerotolerance 21 Oxygen and Growth: Catalase 22 Oxygen and Growth: Oxidase Food and Water Safety 23 Plate Count Treatment and Control of Microbial Growth 24 Killing by Ultraviolet Light 25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method) Microbial Genetics and Genetic Engineering 26 ELISA and Using Nucleotide BLAST (Dry Lab) 27 Analysis of DNA: Electrophoresis 28 Gene Transfer: Transformation Infectious Diseases 29 Parasitic Protozoa 30 Parasitic Worms 31 Transmission: Vectors of Disease 32 Transmission: Epidemiology Activity 33 Vaccination and Herd Immunity Diagnosing Infections 34 Gel Immunoprecipitation (Immunodiffusion) 35 Passive (Indirect) Agglutination 36 Rapid Immunoassays 37 Hemagglutinin Inhibition Assay 38 EnteroPluri-Test Environmental Microbiology 39 Soil Microbiology 40 Nitrate Reduction 41 Viral Titration Hydrolytic Enzymes and Disease Mechanisms 42 DNase Test 43 Gelatin Hydrolysis 44 Starch Hydrolysis 45 Casein Hydrolysis 46 Snyder Agar Biochemical Testing and Diagnosis of Disease 47 Phenol Red Sugar Broth 48 Triple-Sugar Iron Agar 49 Litmus Milk 50 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions 51 Urease Test Putting It All Together 52 Identification of Unknown Bacteria

About the Author :
Steven Obenauf, Ph.D., has been teaching microbiology lectures and laboratories at Broward College since 1993. For six years before moving to the Fort Lauderdale area, he taught medical immunology and medical virology to first- and second-year medical students as well as first-year pharmacy students. His dedication to his students has led him to be a recipient of both Professor of the Year and Endowed Teaching Chair awards for undergraduate teaching and the “Golden Apple” teaching award bestowed by the graduating medical class. Dr. Obenauf is a registered microbiologist and belongs to both the American Society for Microbiology (ASM) and National Association of Biology Teachers. For 10 years, he served as the secretary and newsletter editor for the Florida branch of ASM. He loves using various forms of technology as part of his teaching and has given workshops and mentored other faculty at his college on in-class and online technology tools. Dr. Obenauf is currently Professor of Biological Sciences at Broward College. This manual brings together two of his passions: microbiology and photography Susan Finazzo, M.S., Ph.D., has a diverse academic background with degrees in biology, geology, microbiology, and horticulture. Her background, clinical work experience, and innate interest in the living world have allowed her to teach a broad variety of subjects. Her first academic love has always been microbiology. Throughout the past 20 years, Dr. Finazzo has taught students at both the 2-year college level and university level. She is a member of the National Association of Biology Teachers and has held several leadership positions including serving as president in 2017. She has been nominated for several teaching and service awards, and has twice been the recipient of an Endowed Teaching Award. Dr. Finazzo is very interested in new pedagogical approaches and tools to address the learning styles of the millennial student.


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Product Details
  • ISBN-13: 9781260786095
  • Publisher: McGraw-Hill Education
  • Publisher Imprint: Mcgraw-Hill Education
  • Height: 274 mm
  • No of Pages: 384
  • Spine Width: 20 mm
  • Width: 226 mm
  • ISBN-10: 1260786099
  • Publisher Date: 23 Mar 2021
  • Binding: Spiral bound
  • Language: English
  • Returnable: Y
  • Weight: 739 gr


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Laboratory Manual for Microbiology Fundamentals: A Clinical Approach
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