Engineered Plga Particles for Mucosal Vaccine Delivery
close menu
Bookswagon
search
My Account
Home > Family and health > Coping with personal, social and health topics > Coping with / advice about ageing > Engineered Plga Particles for Mucosal Vaccine Delivery
Engineered Plga Particles for Mucosal Vaccine Delivery

Engineered Plga Particles for Mucosal Vaccine Delivery


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Mucosal immunization has been demonstrated to convey protective immunity both systemically and at the mucosal surface. But in the female reproductive tract, intravaginal (ivag) drug delivery is limited by existing chemical and physical barriers. To achieve efficient delivery to the reproductive epithelium, a vehicle is necessary to protect therapeutic agents from the harsh environment and overcome the mucus gel, a diffusion-limiting barrier. Polymeric particles such as PLGA have been widely explored in drug delivery for their ability to encapsulate and transport various payloads, usually for systemic of subcutaneous delivery (i.e., protein, nucleic acids, and drugs). Furthermore, the surface of these particles can be modified to add functionalities such as increased transport or facilitate cell/tissue-specific uptake in vivo. To explore the application of polymer particles in ivag delivery, we formulated PLGA nanoparticles (d∼ 150--170 nm) that can encapsulate therapeutic payloads and quickly diffuse through the mucus gel. Surface modifications of nanoparticles were achieved by first functionalizing the particle surface with avidin (∼420 molecules/nanoparticle), creating a robust and versatile platform that allows for controlled immobilization of biotinylated-PEG (MW=2, 5, and 10 kDa) at 100% or 10% surface density. This systematic PEGylation produced particle formulations with more net-neutral surface and low aggregation. PEG surface coating facilitates particle diffusion through human cervical mucus with diffusion coefficients up to 3--10x higher than unmodified or avidin-modified particles. For optimally PEGylated particles, diffusion in human cervical mucus is as rapid as diffusion in water. To assess the properties of nanoparticles that are optimal for ivag delivery, the distributions of particles within the reproductive tract of mice were monitored after delivery by quantifying particle residence time, mucus-association, and tissue penetration. Negatively charged PLGA particles (NP) exhibited 5x shorter residence time compared to adhesive (avidin-coated or Avid-NP) or stealth (PEG-coated or PEG-NP) particles. Significant differences in mucus association were observed; higher concentrations of Avid-NP formulations were measured (324 microg nanoparticle) in the lumen compared to NP (216 microg) and PEG-NP (171 microg) after 0.5 hrs. On average, PEG-NP penetrated the tissue more efficiently, especially at a longer time points (6 hrs), where up to 4x higher concentration in the tissue was detected over other formulations. Surface modification of PLGA particles also produced differences in release of protein and nucleic acid payloads. The presence of avidin-palmitate conjugate formed a monolayer coating on the surface of particles that prevented degradation, but also hindered release of protein and DNA (∼2x reduction), and in a pH-dependent manner for DNA payloads (reduced at more acidic pH). This hindrance in release reduced the effectiveness of modified-particles as ivag vaccine delivery vehicles, such that no differences between antibody and IFN-gamma production were observed for animals treated with modified particles compared to untreated animals over 8 weeks. The interplay between efficiency of particle delivery into cell and payload release was further explored in vitro. PLGA particles with BSA on the surface were taken up ∼4x more efficiently and with specificity by OK cells that expresses the receptor megalin. However, due to the lower plasmid DNA (encoding luciferase gene) release from the particles, the resulting luciferase expression was 3--10 fold lower. Our findings demonstrated that systematic...


Best Sellers


Product Details
  • ISBN-13: 9781243782625
  • Publisher: Proquest, Umi Dissertation Publishing
  • Publisher Imprint: Proquest, Umi Dissertation Publishing
  • Height: 246 mm
  • Weight: 318 gr
  • ISBN-10: 1243782625
  • Publisher Date: 01 Sep 2011
  • Binding: Paperback
  • Spine Width: 9 mm
  • Width: 189 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Engineered Plga Particles for Mucosal Vaccine Delivery
Proquest, Umi Dissertation Publishing -
Engineered Plga Particles for Mucosal Vaccine Delivery
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Engineered Plga Particles for Mucosal Vaccine Delivery

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!
    Your IP: 216.73.216.195 IN