Buy Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil
Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil

Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

The relatively simple combinatorial rules responsible for establishing the initial metameric expression of sloppy-paired-1 ( slp1) in two cell wide stripes in the posterior half of each parasegment in the Drosophila embryo make this system an attractive model for investigating the mechanism of regulation by Runt and other pair-rule transcription factors. I analyzed various slp1-lacZ reporter genes in order to identify cis-regulatory sequences responsible for this early pattern. This work identifies two distinct elements, a proximal early stripe element (PESE) and a distal early stripe element (DESE) located from -3.8 kb to -1.8 kb and from -8.7 kb to -6.6 kb upstream of the slp1 promoter, respectively. The distal element expresses both odd and even-numbered stripes with inappropriate expression in the anterior half of the odd-numbered parasegments due to an inability to respond to repression by Even-skipped (Eve). In contrast, the proximal element expresses only even-numbered stripes and mediates repression by Eve as well as by the combination of Runt and Fushi-tarazu (Ftz). A minimal PESE element retains Eve-dependent repression, but is expressed throughout the even-numbered parasegments due to the loss of repression by Runt and Ftz. Importantly, a composite reporter gene containing both cis-elements faithfully recapitulates slp1 regulation in a manner beyond what is expected from combining their individual patterns. I propose a model whereby the metameric expression of slp1 is achieved through Runt dependent regulation of interactions between the DESE and PESE enhancers and the slp1 promoter. The role of DNA-binding by Runt in slp1 regulation was further investigated by examining expression of DESE-lacZ reporters containing mutations in different Runt binding sites. These results indicate these sites are important for repression of DESE-lacZ but are not essential for activation. Complementary studies using a DNA-binding defective form of Runt confirm the importance of DNA-binding for slp1 repression and also reveal a role for DNA-binding by Runt in activation of slp1 and the DESE-lacZ reporter. This work reveals new insights into Runt dependent regulation and opens the door for future studies on the mechanisms underlying the developmental regulation of gene expression.


Best Sellers


Product Details
  • ISBN-13: 9781243747921
  • Publisher: Proquest, Umi Dissertation Publishing
  • Publisher Imprint: Proquest, Umi Dissertation Publishing
  • Height: 254 mm
  • Weight: 277 gr
  • ISBN-10: 1243747927
  • Publisher Date: 01 Sep 2011
  • Binding: Paperback
  • Spine Width: 9 mm
  • Width: 203 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil
Proquest, Umi Dissertation Publishing -
Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Interplay Between Distinct Cis-Regulatory Modules Mediates Combinatorial Regulation by Runt and Other Pair-Rule Transcription Factors During Drosophil

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!