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Laboratory Methods of the United States Army

Laboratory Methods of the United States Army


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About the Book

This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1919 edition. Excerpt: ...agar containing 0.2 per cent. sodium nitrate is sterilized in deep tubes used for agar shake cultures. The nitrate inhibits formation of ga_$ and thus facilitates observation of colony stru_ItuYe-f media should be heated and cooled just before moculatlon to drive out air and facilitate anaerobic conditions 1 Taken from Report N 0. I of the Anaerobe Committee of the Medical Research Committee of Great Britain. April, 1918 II. Material for Examination.--r. Serous exudates from wounds. 2. Infected muscle tissue. 3. Fluid from hemorrhagic blisters. 4. Blood cultures. III. Microscopic Exarnination.--r. Film and H anging-drop Prearations.--Stain film preparations of fresh wound material by Gram s method and examine hanging-drop preparations of infected fluids for the presence of motile organisms. Gram-positive bacilli of any kind in films are to be looked upon as indicating the presence of anaerobes, but do not necessarily warrant a diagnosis of gas gangrene. Motile organisms found under these conditions are B. sporogenes (Metchnikoff) and Vibrion sejbtique. The presence of large non-motile bacilli is indicative of B. welchii. 2. M orjhology.--B. welchii, stout bacillus of varying length, ends rounded, non-sporulating in the presence of fermentable sugars, not infrequently showing capsule. Bacilli are generally single but often are seen in short chains. B. sjborogenes, sporulating bacillus, variable in length, more slender than B. welchii; spores are oval, central or subterminal in position, occasionally absent in very recent wounds. Vibrion septique corresponds roughly in size to B. sjorogenes. Certain morphological variations are characteristic: (a) club-shaped forms; (b) small oval types deeply and uniformly...


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Product Details
  • ISBN-13: 9781234224912
  • Publisher: Rarebooksclub.com
  • Publisher Imprint: Rarebooksclub.com
  • Height: 246 mm
  • No of Pages: 90
  • Spine Width: 5 mm
  • Width: 189 mm
  • ISBN-10: 1234224917
  • Publisher Date: 15 Jan 2013
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 177 gr


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