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Home > Art, Film & Photography > Cell Imaging: Confocal Laser Scanning Microscopy, Green Fluorescent Protein, Digital Holographic Microscopy, Forster Resonance Energy Transfer
Cell Imaging: Confocal Laser Scanning Microscopy, Green Fluorescent Protein, Digital Holographic Microscopy, Forster Resonance Energy Transfer

Cell Imaging: Confocal Laser Scanning Microscopy, Green Fluorescent Protein, Digital Holographic Microscopy, Forster Resonance Energy Transfer


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About the Book

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 31. Chapters: Confocal laser scanning microscopy, Green fluorescent protein, Digital holographic microscopy, Forster resonance energy transfer, Fluorescence microscope, Fluorescence Lifetime Imaging Microscopy, Laser capture microdissection, Two-photon excitation microscopy, Fluorescence recovery after photobleaching, Second harmonic imaging microscopy, Total internal reflection fluorescence microscope, 7-Aminoactinomycin D, Micrographia, Indo-1, RoGFP, Fura-2, Pulse-chase analysis, Calcium imaging, Fura-2-acetoxymethyl ester, Nanovid microscopy, Fluo-4, Time-lapse microscopy, Brainbow, Fluorescence loss in photobleaching, Cameleon, Multiphoton fluorescence microscope, Synapto-pHluorin. Excerpt: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and ex...


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Product Details
  • ISBN-13: 9781157233565
  • Publisher: Books LLC, Wiki Series
  • Publisher Imprint: Books LLC, Wiki Series
  • Height: 246 mm
  • No of Pages: 32
  • Spine Width: 2 mm
  • Weight: 77 gr
  • ISBN-10: 1157233562
  • Publisher Date: 05 Jul 2011
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Sub Title: Confocal Laser Scanning Microscopy, Green Fluorescent Protein, Digital Holographic Microscopy, Forster Resonance Energy Transfer
  • Width: 189 mm


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