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Home > Art, Film & Photography > Fluorescence: Fluorescence Techniques, Fluorescence Correlation Spectroscopy, Chemical Imaging, Forster Resonance Energy Transfer
Fluorescence: Fluorescence Techniques, Fluorescence Correlation Spectroscopy, Chemical Imaging, Forster Resonance Energy Transfer

Fluorescence: Fluorescence Techniques, Fluorescence Correlation Spectroscopy, Chemical Imaging, Forster Resonance Energy Transfer


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About the Book

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 31. Chapters: Fluorescence techniques, Fluorescence correlation spectroscopy, Chemical imaging, Forster resonance energy transfer, Fluorescence microscope, Fluorescence spectroscopy, Vertico SMI, Fluorescence recovery after photobleaching, 4Pi Microscope, Quenching, Stokes shift, Fluorescence cross-correlation spectroscopy, Fluorescence anisotropy, Laser-induced fluorescence, Optode, Supercritical angle fluorescence microscopy, Cold vapour atomic fluorescence spectroscopy, Molecular beacon, Fluorescence loss in photobleaching, Kautsky effect, Advanced Microscopy Group, Nuclear resonance fluorescence, FLEX mission, Fluorescence intermittency, Simulated fluorescence process algorithm, Dexter electron transfer, EDANS, DiI. Excerpt: Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly changing around the average number. The analysis gives the average number of fluorescent particles and average diffusion time, when the particle is passing through the space. Eventually, both the concentration and size of the particle (molecule) are determined. Since the method is observing a small number of molecule in a very tiny spot, it is a very sensitive analytical tool. Both parameters are important in biochemical research, biophysics, a...


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Product Details
  • ISBN-13: 9781156473573
  • Publisher: Books LLC, Wiki Series
  • Publisher Imprint: Books LLC, Wiki Series
  • Height: 246 mm
  • No of Pages: 32
  • Spine Width: 2 mm
  • Weight: 77 gr
  • ISBN-10: 1156473578
  • Publisher Date: 06 Jul 2011
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Sub Title: Fluorescence Techniques, Fluorescence Correlation Spectroscopy, Chemical Imaging, Forster Resonance Energy Transfer
  • Width: 189 mm


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