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Laboratory Work with Mosquitoes

Laboratory Work with Mosquitoes


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About the Book

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1902 Excerpt: ... of filtered sterile salt solution of strength six-tenths of i per cent. The bottle should be stoppered with cotton and the pipette sterilized before use; otherwise bacteria or spores will appear in the salt bottle in a few days, seriously confusing the field of dissection. The mosquito is caught from the cage in a short testtube or small bottle and killed with vapor of chloroform. Half a minute is usually enough. Some will revive if afterwards shaken about in the air, --a kind of "artificial respiration." If chloroform is not at hand, ether, benzine, tobacco smoke, or the cyanide bottle may be used. Legs and wings are cut off close with scissors. If only the stomach is wanted, the abdomen is cut off close to the thorax and laid in a drop of salt solution on the slide. With the needles carefully detach the last two rings from those in front, taking care to touch only the extreme outside of each ring. Then pull the two detached rings backwards from the rest of the mass. By using great gentleness the ovaries and intestine will first appear and finally the Malpighian tubes and stomach. This method, --a very easy and pleasing one, originating, I believe, with Dr. Ronald Ross, --serves to show perfectly all the abdominal structures except the dorsal vessel, which does not bear on our studies. It must be worked out more slowly and laboriously with needles and forceps (Woldert, 13). Under the low power, before putting on the cover, the intestine, oviducts and neck of the stomach will be seen to be in active normal and reverse peristalsis, the contents of the stomach and gut flowing with each contraction backwards and forwards. The field will be at first obscured with turbid fatty fluid from the insect; this fluid must be carefully washed off with an excess ...


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Product Details
  • ISBN-13: 9781155081021
  • Publisher: Rarebooksclub.com
  • Publisher Imprint: Rarebooksclub.com
  • Height: 246 mm
  • No of Pages: 26
  • Spine Width: 1 mm
  • Width: 189 mm
  • ISBN-10: 1155081021
  • Publisher Date: 13 May 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 68 gr


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