Applying Genomic and Proteomic Microarray Technology in Drug Discovery

Microarrays play an increasingly significant role in drug discovery. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. The author addresses the drawbacks, helping you avoid unnecessary pitfalls, and provides practical tips on how to employ the technology in drug discovery and development. The book details the commercial landscape, covering the many issues surrounding the future adoption of gene expression and protein microarrays for pharmacogenomic and pharmacoproteomic applications. The author critically assesses those studies that have helped define applications in genomics and proteomics, explains gene expression microarray applications, and examines the utility of the protein microarray. He covers alternative substrates and the preparation of various surface chemistries together with their suitability for immobilization of nucleic acids and proteins. He delineates the mechanics of microarraying including environmental conditions, printer and pin performance, as well as discussion regarding setting up the print run. The book supplies protocols for printing nucleic acids and proteins and an in-depth discussion of other important parameters such as print buffers (inks) and factors influencing print quality. An understanding of the making of a microarray is fundamentally important to those interested in producing "spotted" arrays and their proper use. As this technology expands in popularity and usefulness, industry experts must grasp the fundamental principles behind it, its strengths, and its limitations. A basic reference for users of microarray technology in drug discovery, this book offers a detailed perspective and insight into the present and future uses of this technology.

Table of Contents:
QUANTITATIVE BIOLOGY: THE "OMICS" ERA Introduction Microarray Format General Utility The Omic Era Role of Gene Expression Microarrays in Drug Discovery Proteomics Today: The Great Challenge Potential Role for Protein Microarrays in Drug Discovery Critical Issues with Protein Microarrays Future medicine: Pharmacoproteomics or pharmacogenomics? References COMMERCIAL MICROARRAYS Introduction In situ arrays Ex situ or spotted arrays 3D and 4D chips Flow-Thru biochips note: flow-thru is a general term when applied here, remove TM. This should have also been corrected in the book proofs. Electronic biochips Future opportunities References SUPPORTS AND SURFACE CHEMISTRIES Introduction Substrates Summary References ARRAYING PROCESSES Introduction Creating spotted microarrays Printing parameters Protocols for printing nucleic acids Protocols for printing proteins References GENE EXPRESSION: MICROARRAY-BASED APPLICATIONS Introduction Applications demonstrating DNA microarray utility Biomedical research applications References PROTEIN MICROARRAY APPLICATIONS Introduction Spot theory Applications demonstrating protein microarray utility Measuring microarray performance Other microarray formats useful for proteomic applications References Index