Lichen-Derived Products: Extraction and Applications

The purpose of this book is to provide reference material that includes current developments along with a future outlook on the topic. It is divided into two sections; "Morphological Overview and Extraction Prospects" and "Trends and Applications". Part I contains four chapters that provide an overview and systematically discuss the physical morphology, suitability and extraction aspects of lichens and their secondary metabolites. Part II includes eight chapters that give in-depth insights on recent and valuable applications of lichen and their obtained products in several applied sectors, including ethnopharmacology, therapeutics, paper and dye, nutraceuticals, cosmetics, herbal industries, etc.

Table of Contents:
Preface xiii Part 1: Morphological Overview and Extraction Prospects 1 1 An Overview on Lichen’s Morphology, Chemistry of Derived Products, and Current Scenario 3 Mohd Yusuf 1.1 Introduction 3 1.2 The Lichen’s Body: Thallus Morphology 4 1.2.1 Foliose 9 1.2.2 Fruiticose 9 1.2.3 Squamulose Lichens 9 1.2.4 Crustose 9 1.2.5 Placodioid/Crustose-Placodioid/Squamulose-Placodioid 9 1.2.6 Leprose 10 1.2.7 Filamentous Lichens 10 1.2.8 Without any Definite Shape or Lichens Without any Visible Thallus 10 1.3 Chemistry of Lichen-Derived Products 10 1.4 Current Scenario of Lichens and Derived Products Thereof 18 1.5 Conclusion and Future Outlook 23 References 24 2 Comparative Perspectives on Extraction Methods for Organic Metabolites and Pollutants from Lichens 27 Patricia Forbes, Leandri van der Wat and Johanna Strumpher 2.1 Introduction 28 2.1.1 Organic Lichen-Derived Compounds 29 2.1.1.1 Organic Air Pollutants 29 2.1.1.2 Lichen Metabolites 29 2.2 Sample Preparation and Solvent Extraction 44 2.3 Extraction of Organic Air Pollutants 45 2.3.1 Soxhlet Extraction 45 2.3.2 Ultrasonic Extraction 46 2.3.3 Microwave-Assisted Extraction 50 2.3.4 QuEChERS 51 2.3.5 Other Extraction Methods 52 2.4 Extraction of Lichen Metabolites 53 2.4.1 Traditional Approaches 54 2.4.1.1 Boiling Extraction 54 2.4.1.2 Shaking 54 2.4.1.3 Ammonia Extraction 54 2.4.2 Alternative Extraction Approaches for Lichen Metabolites 54 2.4.2.1 Soxhlet Extraction 54 2.4.2.2 Ultrasonic Extraction 55 2.4.2.3 Microwave-Assisted Extraction 55 2.4.2.4 QuEChERS 55 2.4.2.5 Solid Phase Microextraction 56 2.4.2.6 Other Extraction Methods 56 2.5 Extract Cleanup 57 2.6 Analysis of Lichen Extracts 59 2.6.1 GC 59 2.6.2 LC 60 2.6.3 Other Analytical Methods 61 2.7 Conclusion 63 References 65 3 Review on Advanced Methods for Extraction and Identification of Nature-Derived Bioactive and Economic Products 75 Sudarshana Deepa Vijaykumar 3.1 Introduction 75 3.2 Accelerated Solvent Extraction 76 3.2.1 Effect of Solubility and Mass Transfer 77 3.2.2 Lowering the Surface Equilibrium 77 3.3 Ultrasound/Ultrasonic Extraction 78 3.4 Microwave Extraction 79 3.4.1 Solvent Composition 81 3.4.2 Solvent Volume 81 3.4.3 Temperature 81 3.4.4 Extraction Time 82 3.4.5 Biomass Characteristics and Water Content 82 3.5 Ultrasound-Microwave Mixed 82 3.6 High Hydrostatic Pressure Extraction 83 3.7 Enzyme Supported Extraction 85 3.7.1 The Types and Characteristics of Enzyme 85 3.7.2 The Breakdown of Cell Walls by Enzyme 86 3.8 Pulsed Electric Field Extraction 87 3.9 Supercritical Fluid Extraction 89 3.9.1 Supercritical Fluid (SCF) 90 3.9.2 Properties of SCF 90 3.10 Modern Identification Methods Based on Spectroscopy Approach 91 3.10.1 Mass Spectroscopy (MS) 91 3.10.2 NMR Spectroscopy 92 3.10.3 UV-Visible Spectroscopy 92 3.10.4 Infrared Spectroscopy 93 3.11 Conclusion 94 References 94 4 Lichens and Lichenology: Historical and Economic Prospects 101 Munesh Sharma and Aash Mohammad 4.1 Historical Prospects of Lichens 101 4.2 Lichens and Symbiosis 103 4.3 Economic Prospects 104 4.3.1 Food Relation 104 4.3.1.1 As Food 104 4.3.1.2 As Fodder 105 4.3.1.3 As Natural Food Flavoring Agent 106 4.3.2 Paper and Textiles Relation 106 4.3.2.1 Litmus Dye Paper 106 4.3.2.2 As Textile Dyes 107 4.3.3 In Perfumery 108 4.3.4 In Medicine and Medical Formulations 110 4.3.5 Age Determination of Substrates/Rocks/Plants or Lichenometric Dating 114 4.3.6 Environmental Benefits/Biomonitoring 115 4.4 Future Outlook and Conclusion 115 References 115 Part 2: Trends and Applications 119 5 Biomedical Aspects of Lichen-Derived Products: Roadmap to Alternative Sources for Synthetic Drugs 121 Anjali Gupta, Pooja Agarwal, Kavita Khatana and Shafat Ahmad Khan 5.1 Introduction 121 5.2 Biological Activities 122 5.2.1 Anticancer Activity 122 5.2.2 Antimicrobial Activity 129 5.2.3 Antiviral Activity 130 5.2.4 Antioxidant Activity 131 5.2.5 Immunomodulator Activity 133 5.2.6 Insecticidal Activity 134 5.3 Conclusion 135 References 136 6 Lichens as Sources of Antibacterial Active Compounds 141 Es-sadeqy Youness, Taha Chouati, Noura Aoussar, Imane Zalegh, Rajaa Ait Mhand, Naima Rhallabi and Fouad Mellouki 6.1 Introduction 142 6.2 Antibacterial Activity of Crude Extracts of Lichens 143 6.3 Antibacterial Activity of Lichenic Acids 153 6.4 Antibacterial Activity of Crude Extracts of Lichens and of Their Bioactive Compounds 159 6.5 Interactions Between Lichen’s Secondary Metabolites and Standard Antibiotics 167 6.6 Future Visions 171 6.7 Conclusion 172 References 172 7 Lichens: Natural Alternatives in Textiles Finishing 179 Amrita Kaushik and Pavan Prakash Giri 7.1 Introduction 179 7.2 Lichens as Dyes 180 7.3 Coloring Components From Lichens 181 7.4 Dye Extraction From Lichens 184 7.5 Advantages and Limitations of Lichens Extracted Dyes 184 7.6 Conclusion and Future Scope of Lichen Dyes 185 References 185 8 A Review on Trends and Opportunity in Edible Lichens 189 Mohd Yusuf 8.1 Introduction 189 8.2 Feed and Fodder 191 8.2.1 Cetraria islandica 191 8.2.2 Roccella montagnei 192 8.2.3 Cladonia rangiferina 193 8.2.4 Bryoria fremontii 193 8.2.5 Parmelia perlata 194 8.2.6 Umbilicaria Species 194 8.2.7 Usnea florida 195 8.3 Lichens as Food Additives 195 8.3.1 Lichen-Based Tea 195 8.3.2 Lichens as Spice 196 8.3.3 Food Coloring Aspects 196 8.4 Lichens as Snuffing Agents 197 8.5 Conclusion and Future Outlook 197 References 198 9 Biotechnological Applications of Lichens 203 Md. Aftab Alam, Rizwana Khatoon, Shamsul Huda, Niyaz Ahmad and Pramod Kumar Sharma 9.1 Introduction 204 9.2 Biotechnological Applications of Lichens 205 9.2.1 Antioxidant Activity 205 9.2.2 Antimicrobial Activity 205 9.2.3 As Bioindicators 206 9.2.4 Anticancer Activity 207 9.2.5 Anti-Insecticidal Activity 210 9.2.6 Enzyme Inhibition Activity 210 9.2.7 Antidiabetic Activity 211 9.3 Conclusion and Future Dimension 212 References 213 10 Potential of Lichen’s Extract as Natural Photosensitizer for Dye-Sensitized Solar Cells 221 Asim Mansha, Sana, Muhammad Usman, Sadia Asim and Matloob Ahmvad 10.1 Introduction 221 10.2 DSSC Operations and Components 224 10.2.1 Operating Principle 224 10.3 Constituents 225 10.3.1 Working Electrode/Semi-Conductor Electrode 225 10.3.2 Electrolyte 226 10.3.2.1 Volatile Organic Solvent 226 10.3.2.2 Room Temperature Ionic Liquid Electrolytes (RTIL) 226 10.3.2.3 Polymer Electrolytes 227 10.3.2.4 Redox Couple 227 10.3.3 Counter Electrode 228 10.4 Natural Dye Sensitizers 229 10.4.1 Chlorophylls 229 10.4.2 Anthocyanins 230 10.4.3 Anthocyanidins 231 10.4.4 Flavonoids 232 10.4.5 Carotenoids 232 10.5 Photoelectrochemical Parameters 234 10.6 Preparation of Dye Solution and Electrodes 239 10.6.1 Preparation of Working Electrode 239 10.6.2 Preparation of CE 240 10.6.3 Preparation of Dye Solution 240 10.7 Conclusion and Future Outlook 241 References 241 11 Lichen-Derived Products as Sustainable Source of Natural Dyes 245 Shahid Adeel, Aqsa Majeed, Fazal-ur-Rehman, Muhammad Azeem, Naeem Iqbal and Nimra Amin 11.1 Introduction 245 11.2 Classification of Lichen 246 11.3 Natural Dyes 247 11.3.1 Classification of Natural Dyes 248 11.3.2 Pros and Cons of Natural Dyes 248 11.3.3 Environmental and Health Aspects of Natural Dyes 249 11.3.4 Economic Aspects of Natural Dyes 250 11.4 Extraction Methods of Natural Dyes 250 11.5 Lichen as a Source of Natural Dye 251 11.5.1 Extraction Methods of Colorants From Lichen 251 11.5.2 Lichen as a Source of Purple Dye 252 11.5.3 Lichen as a Source of Red Dye 253 11.5.4 Lichen as a Source of Yellow Dye 254 11.5.5 Lichen as a Source of Blue Dye 255 11.5.6 Isolation Medium Dependant Colorants from Lichens 255 11.5.7 Future Prospectus of Lichen Dyeing 257 11.6 Conclusion 257 References 258 12 Biomedical Perspectives of Lichen-Derived Products 263 Muhammad Shahid, Arslan Rasool, Fozia Anjum and Muhammad Tjammal Rehman 12.1 Introduction 263 12.2 Coevolution of Lichens 264 12.3 Diversity Among Growth Forms of Lichens 265 12.4 Lichen Secondary Metabolites and Their Structures (Lichen Substances) 266 12.5 Biomedical and Biological Activities of Secondary Metabolites of Lichens 268 12.5.1 Antioxidant Activity 270 12.5.2 Cytotoxic Activity 270 12.5.3 Antiviral Activity 270 12.5.4 Antitumor Activity 271 12.5.5 Antidiabetic Activity of Lichens 271 12.5.6 Antibacterial and Antifungal Activity 272 12.6 Conclusion 272 References 272 Index 277

About the Author :
Mohd Yusuf received his PhD from the Jamia Millia Islamia University, New Delhi, India in 2013. He is now an Assistant Professor at D/O Chemistry, YMD College, M. D. University, Nuh, Haryana, India. He has published more than 50 publications including research and review articles, as well as book chapters in edited volumes. He edited Handbook of Renewable Materials for Coloration and Finishing (Wiley-Scrivener) in 2018.