Handbook of Fluorescent Dyes and Probes

A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 150 FLUORESCENT DYES AND PROBES Handbook of Fluorescent Dyes and Probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, color, material, physical and related allied fields. The ease of use of the handbook is further enhanced by various appendixes provided at the end of the book to conveniently and easily locate the dye as per the reader's need. This is the first book to give the CAS registry numbers, chemical structure, Chemical Abstract (CA) index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient, and quantum yield of fluorescent dyes and probes, as well as to provide access to synthetic procedures (lab scale and industrial scale) of dyes and probes in a single source. This user-friendly handbook also features references on safety, toxicity and adverse effects of dyes and probes on humans, animals and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxocity hematotoxicity hepatotoxicity immunotoxicity marine toxicity microbial toxicity mutagenicity nephrotoxicity neurotoxicity nucleic acid damage oral toxicity phototoxicity phytotoxicity reproductive toxicity skin toxicity Containing imaging/labeling applications, biological/medical applications and industrial applications, Handbook of Fluorescent Dyes and Probes is a convenient, vital resource for industrial and academic researchers, and a valuable desktop reference for medical professionals, lab supervisors, scientists, chemists, biologists, engineers, physicists, intellectual property professionals, students, and professors. Includes all fluorescent dyes & probes known to date and provides a complete, up-to-date library of information in one reference/handbook Includes more than 300 fluorescent dyes & probes organized alphabetically by the commonly used Chemical Name Provides access to synthesis procedures (lab scale and industrial scale) of fluorescent dyes & probes First book to provide references on safety, toxicity and adverse effects of fluorescent dyes and probes on humans, animals, and the environment User-friendly and convenient resource guide for chemical, biological, medical, and intellectual property professionals in a broad range of disciplines

Table of Contents:
PREFACE xv ABOUT THE AUTHOR xvii ACRIDINE HOMODIMER 1 ACRIDINE ORANGE (AO) 3 ACRIDINE ORANGE 10-DODECYL BROMIDE (DODECYL-ACRIDINE ORANGE (DAO)) 10 ACRIDINE ORANGE 10-NONYL BROMIDE (NONYL-ACRIDINE ORANGE (NAO)) 12 ALEXA FLUOR 350 CARBOXYLIC ACID, SUCCINIMIDYL ESTER (AMCA-S) 15 ALEXA FLUOR 430 CARBOXYLIC ACID SUCCINIMIDYL ESTER 17 ALEXA FLUOR 488 CARBOXYLIC ACID SUCCINIMIDYL ESTER 20 ALEXA FLUOR 514 CARBOXYLIC ACID SUCCINIMIDYL ESTER 23 ALEXA FLUOR 532 CARBOXYLIC ACID SUCCINIMIDYL ESTER 25 ALEXA FLUOR 555 CARBOXYLIC ACID SUCCINIMIDYL ESTER 28 ALEXA FLUOR 594 CARBOXYLIC ACID SUCCINIMIDYL ESTER 30 ALEXA FLUOR 633 CARBOXYLIC ACID SUCCINIMIDYL ESTER 33 ALEXA FLUOR 660 CARBOXYLIC ACID SUCCINIMIDYL ESTER 35 ALEXA FLUOR 680 CARBOXYLIC ACID SUCCINIMIDYL ESTER 38 ALEXA FLUOR 700 CARBOXYLIC ACID SUCCINIMIDYL ESTER 40 ALEXA FLUOR 750 CARBOXYLIC ACID SUCCINIMIDYL ESTER 42 ALEXA FLUOR 790 CARBOXYLIC ACID SUCCINIMIDYL ESTER 45 9-AMINO-6-CHLORO-2-METHOXY-ACRIDINE (ACMA) 46 4-AMINO-5-METHYLAMINO-2′,7′-DIFLUOROFLUORESCEIN (DAF FM) 50 4-AMINO-5-METHYLAMINO-2′,7′-DIFLUOROFLUORESCEIN DIACETATE (DAF-FM DA) 52 7–AMINO-4-METHYLCOUMARIN (AMC) (COUMARIN 120) (COUMARIN 440) 54 8-AMINOPYRENE-1,3,6-TRISUL-FONIC ACID, TRISODIUM SALT (APTS) 63 7-ANILINOCOUMARIN-4-ACETIC ACID (ACAA) 65 9-ANTHROYLNITRILE 66 BOBO 1 69 BOBO 3 71 BODIPY FL C5-CERAMIDE (C5-DMB-CERAMIDE) 74 BODIPY FL C5-GANGLIOSIDE GM1 77 BODIPY FL C5-LACTOSYLCERAMIDE 79 BODIPY FL C5-SPHINGOMYELIN (C5-DMB-SPHINGOMYELIN) 81 BODIPY FL C5-SUCCINIMIDYL ESTER 83 BODIPY TR CERAMIDE 85 BO-PRO 1 87 BO-PRO 3 89 4-BROMOMETHYL-6,7-DIMETHOXYCOUMARIN 91 5-(BROMOMETHYL)FLUORESCEIN 93 4-BROMOMETHYL-7-METHOXYCOUMARIN 95 CALCEIN BLUE AM 101 5-CARBOXY-2′,7′-DICHLOROFLUORESCEIN 102 6-CARBOXY-2′,7′-DICHLOROFLUORESCEIN 104 5-CARBOXY-2′,7′-DICHLOROFLUORESCEIN DIACETATE 106 6-CARBOXY-2′,7′-DICHLOROFLUORESCEIN DIACETATE 108 5-CARBOXYFLUORESCEIN 110 6-CARBOXYFLUORESCEIN 114 5-CARBOXYFLUORESCEIN DIACETATE 119 6-CARBOXYFLUORESCEIN DIACETATE 121 5-CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER 125 6-CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER 129 5-CARBOXYNAPHTHOFLUORESCEIN 132 6-CARBOXYNAPHTHOFLUORESCEIN 134 CASCADE BLUE ACETYL AZIDE TRISODIUM SALT 136 CASCADE BLUE HYDRAZIDE TRISODIUM SALT 138 3-CYANO-7-ETHOXYCOUMARIN (7-ETHOXYCOUMARIN-3-CARBONITRILE) 139 3-CYANO-7-HYDROXYCOUMARIN (3-CYANOUMBELLIFERONE) 141 4′,6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE (DAPI) 145 7-DIETHYLAMINOCOUMARIN-3-CARBONYL AZIDE 148 7-DIETHYLAMINOCOUMARIN-3-CARBOXYLIC ACID (DAC) 149 7-DIETHYLAMINOCOUMARIN-3-CARBOXYLIC ACID HYDRAZIDE (DCCH) 153 7-DIETHYLAMINOCOUMARIN-3-CARBOXYLIC ACID SUCCINIMIDYL ESTER (DEAC SE) 155 4-[4-(DIETHYLAMINO)STYRYL]-N-METHYLPYRIDINIUM IODIDE (4-DI-2-ASP) 157 3,3′-DIETHYLOXACARBOCYANINE IODIDE (DIOC2(3)) 160 3,3′-DIHEPTYLOXACARBOCYANINE IODIDE (DIOC7(3)) 163 3,3′-DIHEXYLOXACARBOCYANINE IODIDE (DIOC6(3)) 165 DIHYDRORHODAMINE 123 (DHR 123) 169 DIHYDRORHODAMINE 6G (DHR 6G) 173 6,7-DIMETHOXYCOUMARIN-4-ACETIC ACID 175 7-DIMETHYLAMINOCOUMARIN-4-ACETIC ACID (DMACA) 176 7-DIMETHYLAMINOCOUMARIN-4-ACETIC ACID SUCCINIMIDYL ESTER (DMACA SE) 178 7-DIMETHYLAMINO-4-METHYLCOUMARIN-3-ISOTHIOCYANATE (DACITC) 180 2-[4-(DIMETHYLAMINO)STYRYL]-N-ETHYLPYRIDINIUM IODIDE (DASPEI) 181 4-[4-(DIMETHYLAMINO)STYRYL]-N-METHYLPYRIDINIUM IODIDE (4-DI-1-ASP) (DASPMI) 183 3,3′-DIMETHYL-α-NAPHTHOXACARBOCYANINE IODIDE (JC-9) 186 3,3′-DIPENTYLOXACARBOCYANINE IODIDE (DiOC5(3)) 187 3,3′-DIPROPYLTHIADICARBOCYANINE IODIDE (DiSC3(5)) 189 ETHIDIUM BROMIDE (HOMIDIUM BROMIDE) 193 ETHIDIUM HOMODIMER-1 (ETHD-1) 199 ETHIDIUM HOMODIMER-2 (ETHD-2) 203 FLUORESCEIN 205 FLUORESCEIN DIACETATE (FDA) 213 FLUOROSCEIN-5-ISOTHIOCYANATE (FITC) 219 FLUORESCEIN-5-THIOSEMICARBAZIDE 224 4-HEPTADECYL-7-HYDROXYCOUMARIN (4-HEPTADECYLUMBELLIFERONE) 227 HEXIDIUM IODIDE 229 HOECHST 33258 231 HOECHST 33342 238 HOECHST 34580 244 7-HYDROXYCOUMARIN-3-CARBOXYLIC ACID (UMBELLIFERONE-3-CARBOXYLIC ACID) 246 7-HYDROXYCOUMARIN-3-CARBOXYLIC ACID SUCCINIMIDYL ESTER 250 7-HYDROXY-4-METHYLCOUMARIN-3-ACETIC ACID 252 8-HYDROXY-1,3,6-PYRENETRISULFONIC ACID TRISODIUM SALT (HPTS) (PYRANINE) 253 JOJO 1 263 JO-PRO 1 265 LOLO 1 267 LYSOSENSOR BLUE DND 167 269 LYSOSENSOR BLUE DND 192 271 LYSOSENSOR GREEN DND 153 272 LYSOSENSOR GREEN DND 189 273 LYSOSENSOR YELLOW/BLUE DND 160 (PDMPO) 275 MARINA BLUE (6,8-DIFLUORO-7-HYDROXY-4-METHYLCOUMARIN) (DiFMU) 277 7-METHOXYCOUMARIN-3-CARBONYL AZIDE 280 7-METHOXYCOUMARIN-3-CARBONYL AZIDE 281 7-METHOXYCOUMARIN-3-CARBOXYLIC ACID 282 7-METHOXYCOUMARIN-3-CARBOXYLIC ACID SUCCINIMIDYL ESTER 286 8-METHOXYPYRENE-1,3,6-TRISULFONIC ACID, TRISODIUM SALT (MPTS) 289 NBD C6-CERAMIDE 290 NUCLEAR YELLOW (HOECHST S 769121) 294 OREGON GREEN 488 (2′,7′-DIFLUOROFLUORESCEIN) 297 OREGON GREEN 488 CADAVERINE 300 OREGON GREEN 488 CARBOXYLIC ACID 302 OREGON GREEN 514 CARBOXYLIC ACID 304 OREGON GREEN 488 CARBOXYLIC ACID DIACETATE 306 OREGON GREEN 488 CARBOXYLIC ACID SUCCINIMIDYL ESTER 308 OREGON GREEN 514 CARBOXYLIC ACID SUCCINIMIDYL ESTER 310 OREGON GREEN 488 MALEIMIDE 312 PACIFIC BLUE (6,8-DIFLUORO-7-HYDROXYCOUMARIN-3-CARBOXYLIC ACID) 315 PACIFIC BLUE C5-MALEIMIDE 317 PACIFIC BLUE SUCCINIMIDYL ESTER 318 POPO 1 320 POPO 3 322 PO-PRO 1 324 PO-PRO 3 326 PROPIDIUM IODIDE (PI) 328 1-PYRENEBUTANOIC ACID HYDRAZIDE (PBH) 334 1-PYRENEBUTANOIC ACID SUCCINIMIDYL ESTER 336 1-PYRENEDECANOIC ACID (PDA) 340 1-PYRENEDODECANOIC ACID (PDDA) 343 1-PYRENEHEXADECANOIC ACID 346 1-PYRENEHEXANOIC ACID (PHA) 348 1-PYRENESULFONYL CHLORIDE 350 1,3,6,8-PYRENETETRASULFONIC ACID, TETRASODIUM SALT 352 N-(1-PYRENYL)IODOACETAMIDE 355 N-(1-PYRENYL)MALEIMIDE 357 QSY 7 CARBOXYLIC ACID SUCCINIMIDYL ESTER 361 QSY 9 CARBOXYLIC ACID SUCCINIMIDYL ESTER 364 QSY 21 CARBOXYLIC ACID SUCCINIMIDYL ESTER 366 REDOXSENSOR RED CC-1 369 RESAZURIN SODIUM SALT 370 RHODAMINE 6G (RH 6G) 373 RHODAMINE 110 (RH 110) 380 RHODAMINE 123 (RH 123) 384 5,5′,6,6′-TETRACHLORO-1,1′,3,3′-TETRAETHYLBENZIMIDAZOLOC-ARBOCYANINE IODIDE (JC-1) 389 TETRAMETHYLRHODAMINE ETHYL ESTER PERCHLORATE (TMRE) 394 TETRAMETHYLRHODAMINE METHYL ESTER PERCHLORATE (TMRM) 396 TO-PRO 1 398 TO-PRO 3 400 TO-PRO 5 403 TOTO 1 405 TOTO 3 408 TRUE BLUE (TRUE BLUE CHLORIDE) 411 VITA BLUE 413 YO-PRO 1 415 YO-PRO 3 419 YOYO 1 421 YOYO 3 425 APPENDIX A INDEX OF CAS REGISTRY NUMBERS 427 APPENDIX B INDEX OF ACRIDINES 431 APPENDIX C INDEX OF ANTHRACENES 433 APPENDIX D INDEX OF BORON COORDINATION COMPOUNDS/DYES 435 APPENDIX E INDEX OF COUMARINS 437 APPENDIX F INDEX OF CYANINES/STYRYLS 439 APPENDIX G INDEX OF HETEROCYCLES 441 APPENDIX H INDEX OF PYRENES 443 APPENDIX I INDEX OF XANTHENES 445 INDEX 447

About the Author :
R. W. SABNIS, PhD, is a registered Patent Agent with U.S. Patent and Trademark Office (USPTO). Dr. Sabnis is one of the world’s foremost experts in dyes, inventing world’s first colored bubbles (non-staining) and color changing dye system with many applications. He has immensely contributed to color science and technology for the past 25 years, particularly, dyes for biomedicine, personal care products, health/beauty products, electronics (displays, OLEDs), inks, paints, plastics, textiles and toys/bubbles. He has over 200 publications which include books, book chapters, encyclopedia chapters, patents, reviews, papers, and symposia presentations. He is also an inventor of several US and international patents (issued/published). Dr. Sabnis is the recipient of Pfish Award, Perkin Innovation Award, Grand Innovation Award, Competitive Spirit Award and Best Doctoral Thesis Award. Dr. Sabnis is awarded the Gold Medal for “Outstanding service to the coloration industry” by the Society of Dyers and Colourists, Bradford, U.K. He has written two books on color chemistry, namely, Handbook of Biological Dyesand Stains and Handbook of Acid-Base Indicators.