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Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > Cancer Cytogenetics and Cytogenomics: Methods and Protocols(2825 Methods in Molecular Biology)
Cancer Cytogenetics and Cytogenomics: Methods and Protocols(2825 Methods in Molecular Biology)

Cancer Cytogenetics and Cytogenomics: Methods and Protocols(2825 Methods in Molecular Biology)


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About the Book

This detailed volume explores the frontiers of this new era in cancer cytogenetics and cytogenomics, focusing on establishing a karyotype as an information-based genomic framework, as well as presenting technological platforms for collecting and analyzing data at the genome level. It begins with several conceptual chapters that introduce ideas such as the Genome Architecture Theory, forcefully emphasizing the importance of cytogenomics in the post-genomics era. The book then proceeds with protocols covering both basic and advanced cytogenetic and cytogenomic methods, as well as diverse experiments beyond traditional cytogenetic platforms, and bioinformatics techniques and resources. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date, Cancer Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal guide to the unique power of this area of research in spatial biology and system-integrated genomics.

Table of Contents:
The New Era of Cancer Cytogenetics and Cytogenomics.- Methods of Detection and Mechanisms of Origin of Complex Structural Genome Variations.- A Paradoxical Role for Somatic Chromosomal Mosaicism and Chromosome Instability in Cancer: Theoretical and Technological Aspects.- The Importance of Monitoring Non-Clonal Chromosome Aberrations (NCCAs) in Cancer Research.- Optical Genome Mapping: A Case Study for Machine-Based Platforms in Cytogenetics and Cytogenomics.- Cell Culture and Slide Preparation for Cytogenetic Studies of Hematological Neoplasms.- Chromosome Bandings and Recognition.- Cancer Sample Analysis Utilizing Single-Nucleotide Polymorphism Array and Array Comparative Genomic Hybridization.- Multitarget Fluorescence In Situ Hybridization Diagnostic Applications in Tumors.- High-Resolution FISH Analysis Using DNA Fibers Generated by Molecular Combing.- FISH-Banding for Characterization of Simple and Complex Chromosomal Rearrangements in Cancer.- Profiling Chromosome Topological Features by Super-Resolution 3D Structured Illumination Microscopy.- Quantitative FISHing: Implications for Chromosomal Analysis.- Characterizing Chemotherapy/Radiotherapy-Induced Genome Chaos in Hodgkin’s Lymphoma Patients Using M-FISH.- Tracking Karyotype Changes in Treatment-Induced Drug-Resistant Evolution.- Generation of Polyploid Giant Cancer Cells (PGCCs) from Hey Ovarian Cancer Cells and Analysis of Embryonic Properties.- Single-Cell MTT: A Simple and Sensitive Assay for Determining the Viability and Metabolic Activity of Polyploid Giant Cancer Cells (PGCCs).- Evaluating Chromosome Instability and Genotoxicity Through Single-Cell Quantitative Imaging Microscopy.- Studying the Dynamics of Tunneling Tubes and Cellular Spheres.- Profiling Numerical and Structural Chromosomal Instability in Different Cancer Types.- The Digital World of Cytogenetic and Cytogenomic Web Resources.


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Product Details
  • ISBN-13: 9781071639450
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Height: 254 mm
  • No of Pages: 397
  • Returnable: Y
  • Sub Title: Methods and Protocols
  • ISBN-10: 1071639455
  • Publisher Date: 25 Jun 2024
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Series Title: 2825 Methods in Molecular Biology
  • Width: 178 mm


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Cancer Cytogenetics and Cytogenomics: Methods and Protocols(2825 Methods in Molecular Biology)
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