Heterologous Expression of Membrane Proteins
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Chemical biology > Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)
Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)

Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Table of Contents:
Solubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs.- Membrane Proteins Production in Escherichia coli: Protocols and Rules.- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly.- Heterologous Expression of Membrane Proteins in E. coli.- Overexpression of the ABC Transporter BmrA within Intracellular Caveolæ in Escherichia coli.- Production of Membrane Proteins in Pseudomonas stutzeri.- Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae.- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.- Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p.- Membrane Protein Production in the Yeast, P. pastoris.- Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses.- Membrane Protein Production in Insect Cells.- Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies.- Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies.- The Heterologous Expression and Purification of GPCRs.- Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor.- Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-Like Particles.- Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface.- Tailor the Purification and Analysis of Membrane Proteins with Modular Detergents.- Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.- Detergent-Free Membrane Protein Purification Using SMA Polymer.- Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies.- Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs.- Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations.


Best Sellers


Product Details
  • ISBN-13: 9781071623671
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Series Title: 2507 Methods in Molecular Biology
  • Width: 178 mm
  • ISBN-10: 1071623672
  • Publisher Date: 01 Jul 2022
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 467
  • Returnable: Y
  • Sub Title: Methods and Protocols


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)
Springer-Verlag New York Inc. -
Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Heterologous Expression of Membrane Proteins: Methods and Protocols(2507 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!