Small Non-Coding RNAs
Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)
Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)

Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology.  Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on  troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Table of Contents:
Regulatory Non-coding RNAs: An Overview.- RNA Precipitation.- Quantitative and Qualitative Assessment of Small RNA Preparations.- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection.- Northern Blot Detection of Tiny RNAs.- Stem-loop qRT-PCR Based Quantification of miRNAs.- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation.- Fluorescence In Situ Hybridization Of Small Non-coding RNAs.- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo.- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography.- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP).- Gene Reporter Assays to Study miRNA Function.- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo.- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs.- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma.- Analysis of the RNA and protein complexome by Grad-seq.- µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs.- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT.- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.


Best Sellers


Product Details
  • ISBN-13: 9781071613856
  • Publisher: Springer-Verlag New York Inc.
  • Publisher Imprint: Springer-Verlag New York Inc.
  • Edition: Revised edition
  • Language: English
  • Returnable: Y
  • Series Title: 2300 Methods in Molecular Biology
  • Width: 178 mm
  • ISBN-10: 1071613855
  • Publisher Date: 02 Apr 2021
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 270
  • Returnable: Y
  • Sub Title: Methods and Protocols


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)
Springer-Verlag New York Inc. -
Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Small Non-Coding RNAs: Methods and Protocols(2300 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!