Protocols of Actinomycetes: Microbiology to Gene editing

Many of the natural products have been isolated and characterized from the actinobacteria, for example polyketides, phenazines, peptides, indolocarbarbazoles, and sterols. To explore new antibiotics from actinobacteria, several bioinformatics and synthetic biology tools were developed. This book covers basics to recent protocols for drug discovery from actinobacteria. Features: Discusses the benefits of production of antibiotics and enzymes from actinomycetes in a large scale Covers the synthetic biology approach Describes the strain improvement of actinobacteria Gives information on basic isolation of actinobacteria and modern techniques Covers the applications and metabolic engineering strategies of actinomycetes This book will be helpful for the pharmaceutical industries and researchers to develop new antibiotics from actinobacteria and can be used in support of future research in drug discovery.

Table of Contents:
1. Isolation of Actinomycetota by different methods. 2. Recent Traditional methods for Isolating Diverse Actinobacteria. 3. Isolation of actinobacteria from caves and limestone habitats. 4. Isolation of endophytic actinobacteria from different sources. 5. Sample treatment and Protocols for the cultivation of marine actinomycetes. 6. A protocol for screening, cultivation, and molecular identification of rare and slow-growing actinomycetes with biosynthetic gene clusters. 7. Isolation and Screening of Enzymes from Actinobacteria. 8. RSM-Based Media Optimization for Enzyme Production By Streptomyces. 9. Protocols for the Screening, Extraction, and Purification of the Hydrolytic Enzymes from Marine Actinomycetes. 10. Marine Actinomycetes' Hydrolytic Enzymes: A structural characterization.11. Isolation of Beta-Glucanase Encoding Genes from Actinobacterial Genus Streptomyces Spp. and its Overexpression in E. coli Heterologous System. 12. Isolation and Screening of Enzyme Inhibitors from Marine Actinobacteria. 13. Screening of amylase and lipase enzymes inhibitors from Actinomycetota. 14. Screening and Characterization of Carotinoids Pigment from the Actinobacteria. 15. Screening of Biosurfactant activity of Actinobacteria. 16. Traditional Screening of Actinomycetes for Antibacterial, Antifungal and Antiviral Properties. 17. Isolation and screening of actinobacteria with antibacterial activity and its production through shake flask culture method. 18. Screening of Antimycobacterial property of actinobacteria. 19. Screening actinomycetes for novel antimycobacterial and anti-cancer molecules. 20. In vitro antioxidant protocols for Actinobacteria. 21. Protocol for Antileishmanial activity using marine Actinobacteria. 22. Shining a Light on the Potent Anti-Malarial Activity actinomycete extract: A Comprehensive In Vitro and In Vivo Evaluation. 23. Screening of marine actinobacteria for postprandial anti-diabetic activity In-vitro and In -vivo Methods. 24. Exploring the Larvicidal Activity of actinomycetes Extracts. 25. Evaluation of Actinobacteria's antibiofilm properties using qualitative and quantitative methods. 26. Microplastic degradation by Actinobacteria. 27. Antibacterial and Antibiofilm activity of biosynthesized nanoparticles from Actinomycetes: An alternative, environmental-free, sustainable approach. 28. Extraction and purification of rapamycin from Streptomyces hygroscopicus. 29. Isolation and Identification of Squalene from the ethyl acetate extract of Streptomyces sp. VITGV156 (MCC 4965). 30. Docking and ADME studies. 31. Methods and protocols for the extraction of the genomic DNA, gene cloning, and heterologous expression from the actinomycetes of extreme habitats. 32. Activation of silent genes in actinomycetes by different methods. 33. Multimodal Spectroscopy Based Proteomics and Metabolomics Approach to Explore Actinomycetes. 34. Isolation of DNA and RNA from actinobacteria. 35. Extraction of DNA from Actinomycetota and amplification of the 16S rRNA gene. 36. Optimization of colony PCR for the identification of actinobacteria by sequencing of the 16S rDNA. 37. Identification of actinomycetes by polyphasic taxonomy. 38. Phylogenetic Analysis of Actinomycetes. 39. Genetic profiling of actinobacterial genomes through polymerase chain reaction based techniques. 40. In Silico Analysis of Phylogenetic Relationship and Non-synonymous Polymorphisms in Actinobacteria. 41. Isolation of metagenomic DNA and data analysis. 42. Practical Methodology of Illumina Sequencing Investigating Actinobacteria Genomes. 43. Digital DNA-DNA hybridization for the identification of Actinomycetota species. 44. antiSMASH: Genome-mining and genome-wide analysis of gene clusters in Actinopolyspora species. 45. Transcriptome Analysis of Actinomycetes RNA Sequencing data: A bioinformatics protocol for beginners. 46. Single-cell RNA sequencing of the actinomycetes. 47. Preparation of Streptomyces RNA for transcriptomics. 48. A protocol for CRISPR-Cas9 mediated knockout construction in Actinobacteria. 49. Design of gRNA for DNA-directed RNA polymerase beta subunit (rpoB) gene in Actinomycete. 50. Acute and Chronic Toxicity of Bioactive Compounds from Actinobacteria. 51. Production, extraction, purification and characterization of bioactive derivatives of phthalate ester from Marine Streptomyces sp.VITJS4

About the Author :
L. Karthik has an extensive expertise in Actinobacteria, Synthetic biology and Drug Discovery Strategies and is presently working as a Project Leader, Synthetic Biology at Salem Microbes Pvt. Ltd, Salem, India. He is Top 2% of all scientists in the world (the Stanford University report published by Elsevier on 10th October 2022&2023). He is an elected Fellow member of Linnean Society of London. He did first postdoctoral training at the Shanghai Jiao Tong University, China under the guidance of eminent scientist Prof. Zhiyong Li. Further he has completed his second postdoctoral fellowship in ECUST, Shanghai, China under the guidance of eminent scientist Prof. Lixin Zhang. He received his Ph.D. degree in Microbiology from the VIT University in 2013. He has published around 75 research papers (h index-26), 5 Books and 6 patents and 6 products commercialized. He is a communication committee member of International Chemical Biology Society, USA and President of Society of Chemical and Synthetic biology, India. In 2016, he got Prestigious “Young Scientist Award” from Indian Association of Applied Microbiologists for his outstanding research work. and got Best Research scholar award-2012 from VIT University. He got 200 young scientist-2012 fellowship (DST) to attend international conference. He got Best VIT ALUMNI AWARD-2022 from VIT University, TN, India. He is Associate Editor of Frontiers in Microbiology and Review editor of Frontiers in Marine Science and also Associate Editor of Current Chinese Engineering Science Journal (Bentham Science). Recently, he proved COVID-19 activity of Kabasura Kudineer (Siddha Herbal formulation). During COVID-19 wave, this drug saved lot of life. For this contribution, he got COVID warrior award from SEED Trust, TN, India.