Introduction to Proteomics
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Introduction to Proteomics: Tools for the New Biology

Introduction to Proteomics: Tools for the New Biology


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About the Book

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Table of Contents:
I. Proteomics and the Proteome.- 1. Proteomics and the New Biology.- 2. The Proteome.- II. Tools of Proteomics.- 3. Overview of Analytical Proteomics.- 4. Analytical Protein and Peptide Separations.- 5. Protein Digestion Techniques.- 6. Mass Spectrometers for Protein and Peptide Analysis.- 7. Protein Identification by Peptide Mass Fingerprinting.- 8. Peptide Sequence Analysis by Tandem Mass Spectrometry.- 9. Protein Identification with Tandem Mass Spectrometry Data.- 10. SALSA: An Algorithm for Mining Specific Features of Tandem MS Data.- III. Applications of Proteomics.- 11. Mining Proteomes.- 12. Protein Expression Profiling.- 13. Identifying Protein-Protein Interactions and Protein Complexes.- 14. Mapping Protein Modifications.- 15. New Directions in Proteomics.

Review :
"Professor Daniel Liebler presents a tutorial on mass spectrometry and its use in proteomics. The basics of mass spectrometers and ionization techniques are described, which is important to ascertain what type of mass spectrometer is most particular for a particular study. The ability to use mass spectrometry data to search databases is an important advance for the non-specialist, because it no longer requires the development of the skills to interpret mass spectra. A basic understanding of the fundamental of the search algorithms and their limitations is described in the book. Finally, applications of mass spectrometry to proteomics are described. This book provides and excellent introduction and overview of proteomics for the graduate student of for any biologist interested in understanding the basics of this rapidly evolving area."-From the foreword by John R. Yates, III "...a tutorial of mass spectrometry for the biological sciences in general and more specifically for the field of proteomics,...Although designated as a book for beginners, it will almost certainly find a wider audience. In particular, both academics and researchers working in associated fields with a need to hone their knowledge skills will find much to interest them in this well written book." - Chromatographia "...an excellent overview of this field in an easy-to-read, succcinct format." - Expert Reviews in Proteomics "...there is a wonderful description of mass spectrometry of peptides, both simple, accurate, up-to-date and very pleasant to read....This book can be recommended for people needing an update in mass spectrometry in proteomics..." - Proteomics


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Product Details
  • ISBN-13: 9780896039919
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 203 mm
  • No of Pages: 198
  • Sub Title: Tools for the New Biology
  • ISBN-10: 0896039919
  • Publisher Date: 04 Dec 2001
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Width: 127 mm


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Introduction to Proteomics: Tools for the New Biology
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