Single Nucleotide Polymorphisms
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Single Nucleotide Polymorphisms: Methods and Protocols(v. 212 Methods in Molecular Biology)

Single Nucleotide Polymorphisms: Methods and Protocols(v. 212 Methods in Molecular Biology)


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About the Book

Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Table of Contents:
SNPs: Why Do We Care?Lisa D. BrooksDenaturing High-Performance Liquid ChromatographyAndreas Premstaller and Peter J. OefnerSNP Detection and Allele Frequency Determination by SSCPTomoko Tahira, Akari Suzuki, Yoji Kukita, and Kenshi HayashiConformation-Sensitive Gel ElectrophoresisArupa GangulyDetection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified AssayChinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, and Richard G. H. CottonSNP Discovery by Direct DNA SequencingPui-Yan Kwok and Shenghui DuanComputational SNP Discovery in DNA Sequence DataGabor T. MarthGenotyping SNPs With Molecular BeaconsSalvatore A. E. Marras, Fred Russell Kramer, and Sanjay TyagiSNP Genotyping by the 5'-Nuclease ReactionKenneth J. LivakGenotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide MicroarraysKatarina Lindroos, Ulrika Liljedahl, and Ann-Christine SyvanenQuantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase MinisequencingCharlotta Olsson, Ulrika Liljedahl, and Ann-Christine SyvanenHomogeneous Primer Extension Assay With Fluorescence Polarization DetectionTony M. Hsu and Pui-Yan KwokPyrosequencing for SNP GenotypingMostafa RonaghiHomogeneous Allele-Specific PCR in SNP GenotypingSoren Germer and Russell HiguchiOligonucleotide Ligation AssayJonas Jarvius, Mats Nilsson, and Ulf LandegrenInvader Assay for SNP GenotypingVictor Lyamichev and Bruce NeriMALDI-TOF Mass Spectrometry-Based SNP GenotypingNiels Storm, Brigitte Darnhofer-Patel, Dirk van den Boom, and Charles P. RodiIndex


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Product Details
  • ISBN-13: 9780896039681
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • Returnable: N
  • Sub Title: Methods and Protocols
  • Width: 152 mm
  • ISBN-10: 0896039684
  • Publisher Date: 07 Oct 2002
  • Binding: Hardback
  • Language: English
  • Series Title: v. 212 Methods in Molecular Biology
  • Weight: 1 gr


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Single Nucleotide Polymorphisms: Methods and Protocols(v. 212 Methods in Molecular Biology)
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