GTPase Protocols Book by Ed Manser at Bookstore - Bookswagon
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Molecular biology > GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)
GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)

GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)


     0     
5
4
3
2
1



Available


X
About the Book

Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.

Table of Contents:
General Protocols.- The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases.- Preparation of GTPases for Structural and Biophysical Analysis.- Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes.- Fluorescence Methods in the Study of Small GTP-Binding Proteins.- Specific Methods.- Ras and Rac as Activators of Reactive Oxygen Species (ROS).- Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs.- A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays.- Determination of the Activity of Rho-Like GTPases in Cells.- of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases.- Effects of Rho Family GTPases on Cell-Cell Adhesion.- Cell Motility and Invasion Assays.- Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases.- Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase.- Preparation of Myristoylated Arf1 and Arf6 Proteins.- ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins.- Arf6 and its Role in Cytoskeletal Modulation.- ARF GTPase-Activating Protein 1.- The Use of Permeabilized Cell Systems to Study Nuclear Transport.- Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes.- Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton.

Review :
From the reviews: "The 20 protocols provide detailed procedures for working with most of the known small GTPases of the RAS superfamily. Included are those from RAS, RHO, RAB, ARF, and RAN. In each section, several different approaches are given, including methods for studying downstream effects. Assay methods range from microarray analysis to cell motility measurements. In each case, there is sufficient detail to allow investigators new to the procedure to use them effectively. In addition, the limitations of the procedures are provided as well as useful "notes" to facilitate adoption in the laboratory. A short bibliography accompanies each chapter and provides adequate primary source references."-Doody's Health Sciences Book Review Journal "In GTPase Protocols, Manser and Leung assemble the key protocols required for researchers venturing into this field … . combination of clear practical detail with underlying theory provides the reader with the information required not only to perform the technique but also to interpret the resulting data. … In summary, this is a collection of detailed and precise experimental methods from some of the leading research groups in this field. The authors are to be congratulated on the clarity brought to each technique … ." (Harry Mellor, Journal of Cell Science, Vol. 116 (7-8), 2003)


Best Sellers


Product Details
  • ISBN-13: 9780896039346
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 267
  • Series Title: 189 Methods in Molecular Biology
  • Width: 155 mm
  • ISBN-10: 089603934X
  • Publisher Date: 05 Jun 2002
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: The Ras Superfamily


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)
Humana Press Inc. -
GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

GTPase Protocols: The Ras Superfamily(189 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!