About the Book
The fast-growing field of developmental biology provides a unifying framework that integrates anatomy, physiology, genetics, biochemistry, cellular and molecular biology, as well as evolutionary biology. The number of reference publications that deal specifically with the practical aspects of experimental developmental biology are relatively scarce. Developmental Biology Protocols grows out of this need for a comprehensive laboratory manual that provides the readers with the principles, background, rationale, as well as practical protocols, for studying and analyzing the events of embryonic development. Its highly practical format and wide range of model systems and multidisciplinary experimental techniques attest to the authors' determination to provide a balanced presentation of both background information and actual laboratory details. This three-volume compilation of relevant and useful information will be a well-utilized resource for both the students and teachers of developmental biology at all levels.
Developmental Biology Protocols offers the most comprehensive, cutting-edge collection of contemporary experimental methods available for the study of embryogenesis and development. Written by an interdisciplinary team of leading scientific investigators, this authoritative collection provides step-by-step instructions for successful laboratory execution. Topics range from animal model system acquisition to molecular genetics, and include high resolution imaging, transgenesis, teratology, comparative anatomy and embryology, the characterization of embryonic structure and function, the analysis of cell function, and the regulation of gene activity. In addition to many unique, new experimental protocols, the authors have also utilized a host of experimental approaches used in other disciplines applicable to the study of development. Comprehensive and richly annotated, Developmental Biology Protocols constitutes the gold-standard reference for today's developmental biologists.
Table of Contents:
Contents of Volume 1: Part I. Introduction. Developmental Biology Protocols: Overview I, Rocky S. Tuan and Cecilia W. Lo. Part II. Systems: Production, Culture, and Storage. Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies, Christopher J. Lowe and Gregory A. Wray. Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins, James A. Coffman and Patrick S. Leahy. The Chick Embryo as a Model System for Analyzing Mechanisms of Development, Diana K. Darnell and Gary C. Schoenwolf. Culture of Avian Embryos, Diana K. Darnell and Gary C. Schoenwolf. Ex Ovo Culture of Avian Embryos, Tamao Ono. Culture of Preimplantation Mouse Embryos, Adam S. Doherty and Richard M. Schultz. In Vitro Culture of Rodent Embryos During Early Postimplantation Period, Masahiko Fujinaga. Cryopreservation of Mouse Embryos, Jean Richa. Part III. Developmental Pattern and Morphogenesis. Studying Head and Brain Development in Drosophila, Robert Finkelstein. Bioassays of Inductive Interactions in Amphibian Development, Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, and George M. Malacinski. Gastrulation and Early Mesodermal Patterning in Vertebrates, Gary C. Schoenwolf and Jodi L. Smith. Craniofacial Development of Avian and Rodent Embryos, Brian K. Hall and Tom Miyake. Examination of the Axial Skeleton of Fetal Rodents, Michael G. Narotsky and John M. Rogers. Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach, B. Rush Waller III and Andy Wessels. Part IV. Embryo Structure and Function. Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos, Kersti K. Linask and Takeshi Tsuda. Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos, Carolyn A. Larabell. Whole-Mount Immunolabeling of Embryos by Microinjection: Increased Detection Levels of Extracellular and Cell Surface Epitopes, Charles D. Little and Christopher J. Drake. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos, Robert M. Zucker, E. Sidney Hunter III, and John M. Rogers. Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy, Robert M. Zucker and John M. Rogers. Magnetic Resonance Imaging Analysis of Embryos, Bradley R. Smith. Optical Coherence Tomography Imaging in Developmental Biology, Stephen A. Boppart, Mark E. Brezinski, and James G. Fujimoto. Ultrasound Backscatter Microscopy of Mouse Embryos, Daniel H. Turnbull. Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function, Kersti K. Linask and James C. Huhta. Calcium Imaging and Cell Cell Signaling, Diane C. Slusarski and Victor G. Corces. Acquisition, Display, and Analysis of Digital Three-Dimensional and Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications, Charles F. Thomas and John G. White. Part V. Cell Lineage Analysis. Cell Lineage Analysis: Applications of Green Fluorescent Protein, Magdalena Zernicka-Goetz and Jonathon Pines. Cell Lineage Analysis: X-Inactivation Mosaics, Seong-SengTan, Leanne Godinho, and Patrick P. L. Tam. Retroviral Cell Lineage Analysis in the Developing Chick Heart, Robert G. Gourdie, Gang Cheng, Robert P. Thompson, and Takashi Mikawa. Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos, Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, and Gary C. Schoenwolf. Cell Lineage Analysis: Videomicroscopy Techniques, Paul J. Heid and Jeff Hardin. Cell Lineage Analysis in Xenopus Embryos, Sally A. Moody. Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryos, David J. Kozlowski and Eric S. Weinberg. Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo, Dazhong Sun, C. May Griffith, and Elizabeth D. Hay. Part VI. Chimeras. Transplantation Chimeras: Use in Analyzing Mechanisms of Avian Development, Diana K. Darnell and Gary C.Schoenwolf. Interspecific Chimeras in Avian Embryos, Nicole M. LeDouarin, Francoise Dieterlen-Lievre, Marie-Aimee Teillet, and Catherine Ziller. Quail Chick Transplantation in the Embryonic Limb Bud, Elizabeth E. LeClair and Rocky S. Tuan. Mouse Chimeras and the Analysis of Development, Richard L. Gardner and Timothy J. Davies. Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo, Simon J. Kinder, Seong-Seng Tan, and Patrick P. L. Tam. Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos, Margaret L. Kirby, Harriett Stadt, Donna Kumiski, and Vlad Herlea. Interspecific Mouse-Chick Chimeras, Josiane Fontaine-Perus. Mosaic Analysis in Caenorhabditis elegans, John Yochem, Meera Sundaram, and Elizabeth A. Bucher. Part VII. Experimental Manipulation of Embryos. Local Application of Bone Morphogenic Protein on Developing Chick Embryos Using a Fibrous Glass Matrix as a Carrier, Akira Nifuji and Masaki Noda. Laser Ablation and Fate Mapping, Margaret L. Kirby, Donna Kumiski, Harriett Stadt, and Greg Hunter. Photoablation of Cells Expressing b-Galactosidase, Sheila Nirenberg. Exo Utero Surgery, Valerie Ngo-Muller and Ken Muneoka. Part VIII. Application of Viral Vectors in the Analysis of Development. Methods for Constructing and Producing Retroviral Vectors, Andrea Gambotto, Seon Hee Kim, Sunyoung Kim, and Paul D. Robbins. Retroviral Gene Transduction in Limb Bud Micromass Cultures, N. Susan Stott and Cheng-Ming Chuong. Construction of Adenoviral Vectors, Alan R. Davis, Nelson A. Wivel, Joseph L. Palladino, Luan Tao, and James M. Wilson. Application of Adenoviral Vectors: Analysis of Eye Development, Jean Bennett, Yong Zeng, Abha R. Gupta, and Albert M. Maguire. The Application of Adenoviral Vectors in the Study of Mammalian Cardiovascular Development, Craig S. Mickanin and H. Scott Baldwin. Index. Contents of Volume 2: Part I. Introduction. Developmental Biology Protocols: Overview II, Rocky S. Tuan and Cecilia W. Lo. Part II. Organogenesis. Drosophila as a Genetic Tool to Define Vertebrate Pathway Players, Nancy M. Bonini. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis, Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, and George M. Malacinski. Analysis of Mammary Gland Morphogenesis, Calvin D. Roskelley, Colleen Wu, and Aruna M. Somasiri. Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro, H. Joseph Yost. Craniofacial Development and Patterning, Harold Slavkin, Glen Nuckolls, and Lillian Shum. Craniofacial Skeletel Morphogenesis In Vitro, Roy C. Ogle. Skeletal Morphogenesis, Stefan Mundlos. Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration, David L. Stocum. Retroviral Infection of T-Cell Precursors in Thymic Organ Culture, Lisa M. Spain, Lisa L. Lau, and Yousuke Takahama. Assay for the Isolation of Hepatogenic Factors: Key Molecules in Hepatocyte Formation, Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, and Wouter H. Lamers. Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures, Cheng-Ming Chuong. Apoptosis in Development, Lin Lin and Zahra F. Zakeri. Methods to Detect Patterns of Cell Death in Drosophila, Nancy M. Bonini. Part III. Abnormal Development and Teratology. Mechanisms of Neurulation, Gary C. Schoenwolf and Jodi L. Smith. Neurulation and Neural Tube Closure Defects, Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes, Maria Kolatsi-Joannou, Jennifer Murdoch, and Patricia Ybot-Gonzales. Neural Tube Defects, Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, and Gary C. Schoenwolf. Experimental Manipulation and Morphometric Analysis of Neural Tube Development, Mary E. Desmond and Patricia A. Haas. Isolation of Neuroepithelium and Formation of Minispheres, Mary E. Desmond and Marcia C. Field. Examination of Normal and Abnormal Placentation in the Mouse, Michael R. Blackburn. Palatal Dysmorphogenesis: Palate Organ Culture, Barbara D. Abbott. Palatal Dysmorphogenesis: Quantitative RT-PCR, Gary A. Held and Barbara D. Abbott. Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities, Sheila M. Bell, Claire M. Schreiner, and William J. Scott. Assessment of Laterality Defects in Rodent Embryos, Masahiko Fujinaga. Cardiac Morphogenesis and Dysmorphogenesis: I. Normal Development, Andy Wessels and Roger Markwald. Analysis of Two Aspects of Left Right Patterning of the Vertebrate Heart: Heart Tube Position and Heart Tube Chirality, Alvin J. Chin. Biologically Based Risk Assessment Models for Developmental Toxicity, Christopher Lau and R. Woodrow Setzer. Part IV. Screening and Mapping of Novel Genes and Mutations. Positional Cloning, Lin Xu and Yefu Li. Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse, Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, and Joshua Daniels. PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes, Jian Zhang and Mary Lou King. Analysis of mRNA Expression During Preimplantation Development, Keith E. Latham, Elena De La Casa, and Richard M. Schultz. Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos, Akihiko Shimono and Richard R. Behringer. HPLC-Based mRNA Differential Display, Thomas B. Knudsen. Part V. Transgenesis: Production and Gene Knockout. Production of Transgenic Drosophila, Miki Fujioka, James B. Jaynes, Amy Bejsovec, and Michael Weir. Transgenic Manipulation of the Sea Urchin Embryo, Jonathan P. Rast. Transgenic Zebrafish, Shuo Lin. Production of Avian Chimeras and Germline Transmission, Kristin L. Woods, Scott Schau, Mary Ellen Clark, Jacqueline A. Bonselaar, and Robert J. Etches. Incorporation of Genetically Modified Cells into Chicken Chimeras, Laura D. Zajchowski, S. Mansoor Mohammed, Qingzia Wei, and Robert J. Etches. Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance, Qingzia Wei, Kristin L. Woods, and Robert J. Etches. Nuclear Transplantation and Cloning in Mammals, Keith E. Latham and Mark E. Westhusin. Production of Transgenic Mice, Jean Richa. Production of Transgenic Mice with Yeast Artificial Chromosome, Aya Jakobovits, Bruce T. Lamb, and Kenneth R. Peterson. Gene-Targeting Strategies, Siew-Sim Cheah and Richard R. Behringer. Chimeric Animals and Germline Transmission, Jaspal S. Khillan. Conditional Gene Knockout Using Cre Recombinase, Yunzheng Le and Brian Sauer. Application of Cre/loxP in Drosophila: Site-Specific Recombination and Transgene Coplacement, Mark L. Siegal and Daniel L. Hartl. Index. Contents of Volume 3: Part I. Introduction. Developmental Biology Protocols: Overview III, Tuan and Lo. Part II. Manipulation of Developmental Gene Expression and Function. Ectopic Expression in Drosophila, Wilder. Clonal Analysis in the Examination of Gene Function in Drosophila, Rooke, Theodosiou, and Xu. Application of Antisense Oligonucleotides in Developing Chicken Embryos, Alexander, Barnes, and Tuan. Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development, Oberlender and Tuan. Part III. Analysis of Gene Expression. Gene Expression Analyzed by Ribonuclease Protection Assay, Bennett. Relative RT-PCR, Giambernardi and Klebe. Gene Expression Analysis Using Quantitative RT-PCR and a Multispecific Internal Control, Shire and Legoux. In Situ PCR Detection of HIV Expression in the Human Placenta, Sheikh, Polliotti, and Miller. Gene Expression Analysis by In Situ Hybridization: Radioactive Probes, Wawersik and Epstein. Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification, Moorman, De Boer, Hagoort, Franco, and Lamers. mRNA and Protein Colocalization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry, Tuan. Whole-Mount In Situ Hybridization to Study Gene Expression during Mouse Development, Lowe and Kuehn. Multicolor Whole-Mount In Situ Hybridization, Hauptmann and Gerster. Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunohistochemistry or Histochemistry, Conlon. Visualization of the Expression of Green Fluorescent (GFP)-Linked Proteins, Ayoob, Jean Sanger, and Joseph Sanger. Part IV. Models of Morphogenesis and Development. Monoclonal Antibodies in the Analysis of Embryonic Development, Bedian. Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay, Yao, Kessler. Amphibian Organizer Activity, Niehrs. Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery, Packard, Cox, and Poole. Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration, Newgreen and Murphy. The Chimeric Human/Mouse Model of Angiogenesis, Petitclerc, von Schalscha, and Brooks. Analysis of Embryonic Vascular Morphogenesis, Sato and Bartunkova. Epithelial-Mesenchyme Interactions, Hall. Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning, Ros, Simandl, Clark, and Fallon. Palate Development In Vitro Procedures, Pisano and Greene. Part V. In Vitro Models and Analysis Differentiation and Development. In Vitro Fertilization Heyner, Tucker. Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development, Peters, Chapman, and Soares. Bone Marrow-Derived Mesenchymal Progenitor Cells, Johnstone and Yoo. Identification, Characterization, and Differentiation of Human Prostate Cells, Mehta, Perez-Stable, Roos, and Nadji. Preparation of Chick Striated Muscle Cultures, DiLullo, George-Weinstein, and Gerhart. Study of Skeletal Myogenesis in Cultures of Unsegmented Somitic Mesoderm, Borycki and Emerson. Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Chondrocyte Maturation, DeLise, Stringa, Woodward, Mello, and Tuan. Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells, DeLise and Tuan. Murine Cells, Haas and Tuan. Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation, Majolagbe and Robey. Studying Early Hematopoiesis Using Avian Blastoderm Cultures, Eisenberg. Isolation and Culture of Mouse Germ Cells, De Miguel and Donovan. Cadherin-Mediated Cell-Cell Interactions, Knudsen and Soler. Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Progeins, Underhill and Zhang. Microinjection of Fluorescently Labeled Alpha-Actinin into Living Cells, Jean Sanger, Danowski, and Joseph Sanger. Pax-3 and Vertebrate Development, Epstein. Genetic-Engineered Models of Skeletal Diseases I. Collagen Type X, Jacenko. Genetic-Engineered Models of Skeletal Diseases II. Targeting Mutations into Transgenic Mice Chondrocytes, Garofalo and Horton. Transgenic Mouse Models of Craniofacial Disorders, Liu, Snead, and Maxson.
Review :
We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This is the collection to get before your next grant application is due. - Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done! Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail...these protocols will be an invaluable aid to every geneticist and developmental biologist. - Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany
