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Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA Arrays: Methods and Protocols(170 Methods in Molecular Biology)
DNA Arrays: Methods and Protocols(170 Methods in Molecular Biology)

DNA Arrays: Methods and Protocols(170 Methods in Molecular Biology)


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About the Book

Microarray technology provides a highly sensitive and precise technique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be rapidly analyzed. The protocols collected in this important volume provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological sample for analysis. Opening with an overview from Professor Southern on the history of DNA array technology, the reader is provided with an all-encompassing manual of the latest state-of-the-art techniques including descriptions of unique ways to synthesize arrays using the photolithographic approach, the synthesis and use of modified monomers, genotyping using DNA Mass Array methodology, as well as information on antisense reagents, a future drug market that will be used to study the effects of these molecules by using array hybridization. Also covered are the business aspects of biochip technology as well as the ethical issues related to genetic analysis. Comprehensive and state-of-the-art, this book will be useful to all levels of scientists from novices to those intimately familiar with array technology.

Table of Contents:
DNA Microarrays.- Gel-Immobilized Microarrays of Nucleic Acids and Proteins.- Sequencing by Hybridization Arrays.- Ethical Ramifications of Genetic Analysis Using DNA Arrays.- Photolithographic Synthesis of High-Density Oligonucleotide Arrays.- Automated Genotyping Using the DNA MassArray™ Technology.- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules.- Printing DNA Microarrays Using the Biomek® 2000 Laboratory Automation Workstation.- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays.- Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays.- DNA Sequencing by Hybridization with Arrays of Samples or Probes.- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents.- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray.- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays.- Use of Bioinformatics in Arrays.- Confocal Scanning of Genetic Microarrays.- Business Aspects of Biochip Technologies.

Review :
"DNA Arrays - Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism. ... The main focus is on a very detailed presentation of the available methods in microarray technology. Experiments are explained carefully and systematic attention is paid to possible variations and particular difficulties that may arise. ...Useful tips are offered: as to how to perform direct comparisons of gene-expression patterns between two tissues or two different experimental condition... this book will surely be viewed as one that represented a stepping stone into development of the technique."- Department of Chemistry, University of Toronto "DNA Arrays: Methods and Protocols is both a useful introduction to DNA microarray technology as well as a handy source of protocols for specific applications of the technique. The book covers a broad range of topics related to DNA microarrays beginning of DNA arrays to the analysis of the data that is produced....This book offers many practical insights into DNA microarray analysis that should prove to be useful to scientists interested in implementing this technique in their research....the reader should be able to easily follow the progression of the material through the different stages one would need to perform to design a microarray experiment. This book will be of interest for those readers seeking practical information about DNA array methodologies." - Biotech Software & Internet Report "...of interest to postgraduate students and postdoctoral researchers who have begun work on DNA arrays and need a broader view of the field, together with useful tips for solving practical problems." - Angewente Chemie International "DNA Arrays-Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism." -The Analyst "...a comprehensive guide to DNA microarrays...provides a starting point where one can begin to tackle the vast amount of new literature on the subject." -The National Journal of India


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Product Details
  • ISBN-13: 9780896038226
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 268
  • Series Title: 170 Methods in Molecular Biology
  • Width: 155 mm
  • ISBN-10: 089603822X
  • Publisher Date: 16 Mar 2001
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Methods and Protocols


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