Angiogenesis Protocols
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Angiogenesis Protocols: (v. 46 Methods in Molecular Biology)

Angiogenesis Protocols: (v. 46 Methods in Molecular Biology)


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About the Book

Several anti-angiogenic agents are now in clinical trials for a range of diseases characterized by uncontrolled blood vessel formation, including cancer and diabetic retinopathy. The potential value of anti-angiogenic agents has given researchers in this field new enthusiasm and impetus. This volume will provide a set of up-to-date reviews on the important components of this process coupled with a set of critical chapters describing the various techniques for studying angiogenesis. In addition, several state-of-the-art chapters will describe associated techniques such as the isolation of microvascular endothelial cells, and the basic principles behind the use of transgenic mice in the study of angiogenesis. The book will provide not only 'recipes' for the study of angiogenesis, but also an up-to-date account of the biology of angiogenesis.

Table of Contents:
I. Special Review Article. Therapeutic Inhibition of Angiogenesis, Hua-Tang Zhang and Roy Bicknell. II. Angiogenesis Protocols In Vivo. Microscopic Assessment of Angiogenesis in Tumors, Stephen B. Fox. In Vivo Matrigel Migration and Angiogenesis Assays, Katherine M. Malinda. Alginate Microbead Release Assay of Angiogenesis, Clifford Y. Ko, Vivek Dixit, William W. Shaw, and Gary Gitnick. Disc Angiogenesis Assay, Anthony C. Allison and Luis-F. Fajardo. Sponge Implant Model of Angiogenesis, Silvia P. Andrade. Hollow Fiber Assay for Tumor Angiogenesis, Roger M. Phillips and Michael C. Bibby. Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy, Axel Sckell and Michael Leunig. Angiogenesis Assays Using Chick Chorioallantoic Membrane, David C. West, W. Douglas Thompson, Paula G. Sells, and Mike F. Burbridge. Corneal Assay for Angiogenesis, Marina Ziche. III. Angiogenesis Protocols In Vitro. Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting, Ana M. Schor, Ian Ellis, and Seth L. Schor. Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis, Ana M. Schor, Ian Ellis, and Seth L. Schor. Rat Aortic Ring: 3D Model of Angiogenesis In Vitro, Mike F. Burbridge and David C. West. In Vitro Matrigel Angiogenesis Assays, M. Lourdes Ponce. IV. Associated Techniques. Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture, Peter W. Hewett. Transfection and Transduction of Primary Human Endothelial Cells, Stewart G. Martin. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization, Richard C. M. Siow and Jeremy D. Pearson. Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification, Ramesh C. Nayak and Ira M. Herman. Index.

Review :
There is an excellent overview of existing inhibitors of angiogenesis, including both natural and man-made and a review of the biological features that define efficacy and specificity of anti-angiogenic therapies. Most existing in vivo angiogenesis assays are combined in one edition. New microvessel markers are listed to allow more accurate microvessel counts. The details on assays that were proprietary only to a few labs nationwide, such as dorsal skinfold, chamber, and corneal neovascularization assays, are now available to the general science community. Given the well known difficulties of transfection of the primary endothelial cultures, the chapter summarizing information available on transfection and transduction of these cells is particularly useful...This is the first book offering such a variety of angiogenesis assays to the general reader where the scientific basis is given in accessible language and technical procedures are readily available. Its publication will undoubtedly facilitate new experiments and further therapeutic angiogenesis. - Doody's Health Sciences Book Review Journal The book provides an excellent review concerning anti-angiogenesis. Inhibiting angiogenesis is an important topic in cancer control...the book would be a convenient reference for any investigator or laboratory interested in angiogenesis. Angiogenesis Protocols is recommended to any research orientated health science library. - E-Streams Electronic Book Review Journal Timely and highly practical, Angiogenesis Protocols is an important resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources." - Anticancer Research


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Product Details
  • ISBN-13: 9780896036987
  • Publisher: Humana Press Inc.
  • Binding: Hardback
  • Language: English
  • Series Title: v. 46 Methods in Molecular Biology
  • Width: 229 mm
  • ISBN-10: 0896036987
  • Publisher Date: 16 Mar 2001
  • Height: 152 mm
  • Returnable: N
  • Weight: 605 gr


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Angiogenesis Protocols: (v. 46 Methods in Molecular Biology)
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