In Situ Hybridization Protocols: (No. 123 Methods in Molecular Biology)

Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques - many not covered in the first edition - and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.

Table of Contents:
Section 1. Chromosomal Techniques. Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids DNA-Protein In Situ co- visualization for Chromosome Analysis Radioactive In Situ Hybridization to Animal Chromosomes Native Polytene Chromosomes of Drosophila Melanogaster for Light and Electron Microscopic Observation of the Conformation and Distribution of Molecules Use of Released Chromatin or DNA Fiber Preparations for High-resolution Fiber FISH In Situ Hybridization to Polytene Chromosomes of Drosophila Melanogaster and Other Dipteran Species In Situ Hybridization to Polytene Chromosomes Comparative Gene Mapping in Exotic Species Using FISH Section 2. Tissue Techniques. In Situ Hybridization with Non-radioactive Probes Quantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Heterocellular Systems Comparison of Isotopic and Non-isotopic Labeling Methods for In Situ Hybridization of Tissue Sections The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Co-localise mRNA and Protein in Tissue Sections In Situ Hybridization Protocols for Detection of Virus DNA Using Radioactive and Non-radioactive Probes Signal Amplification for DNA and mRNA In Situ Hybridization In Situ Localization of PCR-amplified DNA and cDNA Ultrastructural Detection of Nucleic Acids on Thin Sections of Tissue Embedded in Hydrophilic Resin In Situ Hybridization for Electron Microscopy In Situ Hybridization of Wholemount Embryos In Situ End-labeling of Fragmented DNA and the Localization of Apoptosis Section 3. Appendices/Applications. Molecular Cytogenetic Analysis of Sperm from Infertile Males Undergoing Intracytoplasmic Sperm Injection (ICSI) Localization of HIV-1 DNA and TNF-a mRNA in Human Brain Using PCR In Situ Hybridization (IS/PCR) and Immunocytochemistry

Review :
From Reviews of the First Edition by Andy Choo... "...a very helpful manual...essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire." - - Molecular Biotechnology " ...a good book for anyone wishing to exploit all that in situ hybridization has to offer." - - Biopharm Manufacturing Reviews for Second Edition This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both. - Heredity