Clinical Applications of Capillary Electrophoresis: (27 Methods in Molecular Medicine)

Stephen M. Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including serum and urine proteins, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, and cathepsin D. The protocols offered for DNA analysis include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microstaellite instability in cancers. Many of the methods can be automated to replace more labor intensive and costly tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods.

Table of Contents:
Clinical Applications of Capillary Electrophoresis.- Serum Protein Electrophoresis.- Urine Proteins.- Electrophoresis of Cerebrospinal Fluid.- Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine.- Analysis and Classification of Serum Cryoglobulins.- Myoglobin Analysis.- Enzyme Analysis.- Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis.- Laboratory Diagnosis of Structural Hemoglobinopathies and Thalassemias by Capillary Isoelectric Focusing.- Serum Apolipoproteins.- Gene Dosage in Capillary Electrophoresis.- Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection.- Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis.- Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophoresis.- Serum Lamotrigine Analysis.- Acetonitrile Stacking.- Confirmation of the Presence of Drugs of Abuse in Urine.- Steroid Analysis by Micellar Electrokinetic Capillary Chromatography.- Determination of Polyamines by Capillary Electrophoresis.- Urinary Oxalate and Citrate.- Plasma Nitrite and Nitrate Determination.

Review :
"...This is a very practical handbook for clinical chemists since it covers applications of CE in most 0 prominent areas of this field...In this book the separation conditions are usually clearly described throughout the text...The book is a well written and easy to use source of practical assays for clinical chemists..."-Chromatographia "The content is very wide ranging. There are chapters on protein determinations in different biological fluids, different aspects of DNA analysis as well as enzyme assays and determination of exogenous species such as drugs of abuse. Each chapter provides a summary of different methodology that has been used and places CE in this context....the book contains a wealth of information and should be very useful as a reference for scientists in the clinical laboratory who wish to evaluate CE as an alternative to existing analytical methods." -Elsevier "...not only is the first of its kind, but also is the first book dedicated solely to clinical analysis by CE." - Clinical Chemistry "This issue of Methods in Molecular Medicine is an interesting, well-edited volume about the clinical application of capillary electrophoresis, which aims to give encouragement and guidance to laboratory practitioners new to CE and to demonstrate that the wide range of assays now available means that it is a technique that has something to offer every clinical laboratory."Haematologia "...In marked contrast to conventional electrophoresis, on which there are relatively few books,...for those clinical chemistry laboratories starting t o use CE, this will be a useful book bringing together much information into one easily used volume to keep by the instrument and from which to gain useful instruction."- Annals of Clinical Biochemistry