About the Book
This volume presents an outstanding collection of state-of-the-art methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation and technology - probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC - and are adaptable to a wide range of applications. The laboratory-tested free-radical assays described here in detail will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
Table of Contents:
Part I.Techniques for the Measurement of Oxidative Stress. Oxygen Consumption Methods: Xanthine Oxidase; and Lipoxygenase, Istvan Stadler. Spin Trapping and Electron Paramagnetic Resonance Spectroscopy, Daniel J. Brackett, Gemma Wallis, Michael F. Wilson, and Paul B. McCay. In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy, Gerald M. Rosen, Sovitj Pou, and Howard J. Halpern. Single Photon Counting, Yoshinori Mizuguchi. Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence, Kuldip Thusu, Ehad Abel-Rahman, and Paresh Dandona. Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography,Yorihiro Yamamoto, Yasuhiro Kambayashi, and Takako Ueda. Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membarne Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid,Valerian E. Kagan, Vladimir B. Ritov, Yulia Y. Tyurina, and Vladimir A. Tyurin. Aromatic Hydrozylation: Salicylic Acid as a Probe for Measuring Hydroxyl Radical Production, Andrea Ghiselli. Simple Assay for the Level of Total Peroxides in Serum or Plasma, Kunio Yagi. Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma, Kunio Yagi. Antibodies Against Malondialdehyde-Modified Proteins: Induction and ELISA Measurements of Specific Antibodies, Yves Chancerelle, J. Mathieu, and J. F. Kergonou. Oxidized LDL and Lp(a): Preparation, Modification, and Analysis, Jan Galle and Christoph Wanner. Oxidized and Unoxidized Fatty Acyl, Esters Odile Sergent and Josiane Cillard. Synthesis of Lipid and Cholesterol Hydroperoxide Standards, Donald Armstrong and Richard W. Browne. Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids by High-Pressure Liquid Chromatography, Richard W. Browne and Donald Armstrong. Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo, Kazumasa Aoyagi, Sohji Nagase, Akio Koyama, Mitsuharu Natitam, and Shizuo Tojo. Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC,Thomas M. Wengenack, J. Randall Slemmon, and J. Mark Ordy. Electrochemical Detection of 8-Hydroxy -2- Deoxyguanosine Levels in Cellular DNA, Thomas M. Nicotera and Sofia Bardin. Nitric Oxide Synthase, Ahmad Aljada and Paresh Dandona. Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR, Nader G. Abraham. A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity,Thomas M. Nicotera and Glenn Spehar. Autofluorescent Ceroid/Lipofuscin, Dazhong Yin and Ulf Brunk. In Vivo Technique for Autofluorescent Lipopigments, Francois C. Delori and C. Kathleen Dorey. An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism, Luigia Rossi and Mauro Magnani. Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion, Lucian Saucan. Part II.Techniques for the Measurement of Antioxidant Activity. Simultaneous Determination of Serum Retinol, Tocopherols and Carotenoids by HPLC, Richard W. Browne and Donald Armstrong. EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals; of Vitamin E: Interactions with Vitamin C, Valerian E. Kagan and Nikolai V. Gorbunov. Nonvitamin Plasma Antioxidants, Nicholas J. Miller. Regulatory Antioxidant Enzymes, C. E. Pippenger, Richard W. Browne, and Donald Armstrong. In Vitro Screening for Antioxidant Activity, Donald Armstrong, Tadahisa Hiramitsu, and Takako Ueda. Antioxidant Activity of Low-Density Lipoprotein, Nicholas J. Miller and George Paganga. Lipoic Acid as an Antioxidant: The Role of Dihydrolipoamide Dehydrogenase, Mulchand S. Patel and Young Soo Hong. Reduced Glutathione and Glutathione Disulfide, Richard W. Browne and Donald Armstrong. Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples, Scott Graves, Marianna Sikorska, Henryk Borowy-Borowski, Rodney J. H. Ho, Tot Bui, and Clive Woodhouse. Part III. Techniques Applicable to Both Oxidative Stress and Antioxidant Activity. A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro, Minoru Nakano, Takashi Ito, and Tadahisa Hiramitsu. Analysis of Oxidation and Antioxidants Using Microtiter Plates, German Camejo, Boel Wallin, and Mervi Enojarvi. Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy, Hiroki Haraguchi, Eiji Fujimori, and Kazumi Inagaki. Energy Dispersive X-Ray Microanalysis, Don A. Samuelson. Pro-Oxidant and Antioxidant Assays of Estrogens Joachim G. Liehr and Deodutta Roy. Artificial Radical Generating and Scavenging Systems: Photo-Fenton Reagent and Caged Compounds, Seiichi Matsugo and Ken Fujimori. Index.
Review :
"...the value of this book lies in the effort by the editor to help collect in one volume useful protocols and methods that will give beginners, as well as veterans in the field, a place to start for study of free radicals and antioxidants." -Analytical Biochemistry "...this is a collection of methods whose acquisition would certainly prove useful to those working in the field of oxidative damage and which should provide plenty of ideas for developments and improvements in research methods in this field."-Angew. Chem. Inst. Ed. "...will be a considerable help to all experimenters, especially those seeking to explore a complex system and identify the species coming into play from the initial stage of oxidants stress, i.e. the generation of the primary ROS, to the formation of secondary radicals and intermediates, the action and regulation of defense systems, and eventually to the termination of the oxidative reaction...I can but highly recommend the book to all - beginners and experienced lab hands alike - who work in the field of oxidative stress, ROS and antioxidants." - Folia Microbiologica
