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Home > Mathematics and Science Textbooks > Biology, life sciences > Molecular biology > Plant Gene Transfer and Expression Protocols: (49 Methods in Molecular Biology)
Plant Gene Transfer and Expression Protocols: (49 Methods in Molecular Biology)

Plant Gene Transfer and Expression Protocols: (49 Methods in Molecular Biology)


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About the Book

In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only a complete collection of mainstream methods, but also methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. There are also methods for introducing and analyzing plant genes in Xenopus and heterologous yeast hosts. Plant Gene Transfer and Expression Protocols is an indispensable tool for scientists working in both fundamental (biochemical, physiological, and genetic) and applied aspects of plant biotechnology, plant breeding, agriculture, and horticulture. It provides concise practical help that produces the desired results even for beginners and opens the doors to new research possibilities.

Table of Contents:
Agrobacterium-Mediated Transformation.- Tools for Expressing Foreign Genes in Plants.- of Cloning Plasmids into Agrobacterium tumefaciens.- Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco.- Agobacterium rhizogenes as a Vector for Transforming Higher Plants.- Agrobacterium-Mediated Transformation of Arabidopsis thaliana.- Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues.- Direct Gene Transfer.- Stable Transformation of Barley via Direct DNA Uptake.- Gene Transfer into Plant Protoplasts by Electroporation.- Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues.- Use of Reporter Genes.- The ?-Glucuronidase (gus) Reporter Gene System.- Chloramphenicol Acetyl Transferase Assay.- NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants.- Study of Gene Organization by Southern Blotting and Inverse PCR.- Gene Characterization by Southern Analysis.- Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification.- RNA Techniques for Studying Gene Expression.- Isolation of Whole Cell (Total) RNA.- Poly(A)+RNA Isolation.- In Vitro Translation.- Northern Analysis and Nucleic Acid Probes.- Nuclear “Run-On” Transcription Assays.- RNase A/T1 Protection Assay.- Primer Extension Assay.- Applications of RT-PCR.- In Vitro Transcription of Class II Promoters in Higher Plants.- Analysis of Plant Gene Expression by Reverse Transcription-PCR.- In Situ Hybridization to Plant Tissue Sections.- Xenopus Oocytes as a Heterologous Expression System.- Heterologous Expression in Yeast.- Techniques for Studying Chloroplast Gene Expression.- The Isolation of Intact Chloroplasts.- In Vitro Protein Import by IsolatedChloroplasts.- Targeting of Foreign Proteins to the Chloroplast.- Techniques for Studying Mitochondrial Gene Expression.- Isolation of Mitochondria.- Mitochondrial Nucleic Acid Purification and Analysis.- In organello Protein Synthesis.- Immunological Detection of Proteins.- Separation of Plant Proteins by Electrophoresis.- Western Blotting Analysis.- ELISA Detection of Foreign Proteins.- Immunocytochemical Localization of Proteins.

Review :
The chapters were written by outstanding scientists and [the book] can be recommended to any laboratory working with plants, providing tips and alternatives in case of problems. -Journal of Plant Physiology


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Product Details
  • ISBN-13: 9780896033214
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 229 mm
  • No of Pages: 466
  • Series Title: 49 Methods in Molecular Biology
  • ISBN-10: 089603321X
  • Publisher Date: 27 Sep 1995
  • Binding: Spiral bound
  • Language: English
  • Returnable: Y
  • Width: 152 mm


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Plant Gene Transfer and Expression Protocols: (49 Methods in Molecular Biology)
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