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Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Protein Stability and Folding: Theory and Practice(40 Methods in Molecular Biology)
Protein Stability and Folding: Theory and Practice(40 Methods in Molecular Biology)

Protein Stability and Folding: Theory and Practice(40 Methods in Molecular Biology)


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About the Book

In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

Table of Contents:
Noncovalent Forces Important to the Conformational Stability of Protein Structures.- Degradative Covalent Reactions Important to Protein Stability.- Fluorescence Spectroscopy.- Ultraviolet Absorption Spectroscopy.- Circular Dichroism.- Infrared Spectroscopy.- Identifying Sites of Posttranslational Modifications in Proteins Via HPLC Peptide Mapping.- Urea and Guanidine Hydrochloride Denaturation Curves.- Differential Scanning Calorimetry.- Disulfide Bonds in Protein Folding and Stability.- Solvent Stabilization of Protein Structure.- Site-Directed Mutagenesis to Study Protein Folding and Stability.- Hydrogen Exchange Techniques.- Protein Folding Kinetics.- Molten Globules.- Chaperonin-Assisted Protein Folding of the Enzyme Rhodanese by GroEL/GroES.

Review :
...very useful for any biochemist who needs to understand concepts of protein folding and stability and how protein folding can be monitored and studied. This is a good book for student researchers and should be considered as an addition to courses on protein structure.-Doody's Health Sciences Book Review Journal


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Product Details
  • ISBN-13: 9780896033016
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 377
  • Series Title: 40 Methods in Molecular Biology
  • Width: 155 mm
  • ISBN-10: 0896033015
  • Publisher Date: 24 Mar 1995
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Sub Title: Theory and Practice


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