Biomembrane Protocols
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Molecular biology > Biomembrane Protocols: I. Isolation and Analysis(19 Methods in Molecular Biology)
Biomembrane Protocols: I.  Isolation and Analysis(19 Methods in Molecular Biology)

Biomembrane Protocols: I. Isolation and Analysis(19 Methods in Molecular Biology)


     0     
5
4
3
2
1



International Edition


X
About the Book

Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the isolation and compositional analysis of membranes and address animal, plant, and microbial cells. The topics covered in this first volume include the isolation of subcellular fractions, centrifugation, the extraction and quantitation of lipids, the separation and analysis of proteins, and the isolation and characterization of glycosylated molecules, glycoproteins, proteoglycans, and glycolipids.

Table of Contents:
The Identification of Subcellular Fractions from Mammalian Cells.- The Isolation of Nuclei and Nuclear Membranes from Rat Liver.- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver.- Continuous-Flow Electrophoresis.- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells.- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver.- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients.- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components.- Isolation of Membranes from Tissue Culture Cells.- The Isolation of Membranes from Bacteria.- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts.- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells.- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption.- Separation and Analysis of Phospholipids by Thin Layer Chromatography.- Analysis of Phospholipids by High Performance Liquid Chromatography.- Measurement of Cholesterol in Membranes.- Analysis of Fatty Acids by Gas Liquid Chromatography.- Chemical Assays for Proteins.- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis.- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins.- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase.- The Isolation of Membrane Proteoglycans.- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans.- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration.- The Extraction and Analysis of Glycosphingolipids.

Review :
...of the greatest interest for biochemists, cellular and molecular biologists. -Cellular and Molecular Biology


Best Sellers


Product Details
  • ISBN-13: 9780896032361
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 314
  • Series Title: 19 Methods in Molecular Biology
  • Width: 155 mm
  • ISBN-10: 0896032361
  • Publisher Date: 20 Aug 1993
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: I. Isolation and Analysis


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Biomembrane Protocols: I.  Isolation and Analysis(19 Methods in Molecular Biology)
Humana Press Inc. -
Biomembrane Protocols: I. Isolation and Analysis(19 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Biomembrane Protocols: I. Isolation and Analysis(19 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!