Buy Detecting Genes Book by Eric D. Green - Bookswagon
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Book 1
Book 2
Book 3
Home > Mathematics and Science Textbooks > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)
Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)

Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

Assuming only a basic knowledge of molecular biology, this is the second in a series of manuals which explains how to clone, manipulate, analyze and sequence large segments of DNA, and relate expressed sequence to phenotypic variation. The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure, and solutions.

Table of Contents:
Strategies for gene discovery in mammalian systems; gene identification; mapping strategies; isolation of transcribed sequences; proving a candidate gene is casually mutated; future developments. Constructing and screening normalized cDNA libraries: primary uses and applicatons for cDNA libraries; overview of the methods for synthesizing double-stranded cDNA; cloning vectors for construction of cDNA libraries; overview of the procedure for constructing directionally cloned cDNA libraries; problems commonly encountered in cDNA libraries; cloning efficiencies required to ensure that a representative cDNA library is obtained; theoretical considerations in normalizing cDNA populations; overview of the methods for constructing normalized cDNA libraries; methods for isolating RNA - protocols; construction of oligo (dt)-pimed, directionally clone cDNA libraries in phagemid vectors - protocols; normalization of directionally cloned cDNA libraries - protocols; screening normalized cDNA libraries constructed in phagemid vectors. Direct cDNA. Selection: overview of direct cDNA selection; choosing sources of cloned genomic DNA and cDNA for direct cDNA selection; preparation of biotinylated, clone genomic DNA - protocols; preparation of cDNA - protocols; hybridization reactions and enrichment of the cDNA - protocols; confirming that cDNA products arise from transcriptionally active genes in the genomic region of interest. Exon trapping: strategies for trapping intact exons; protocols for trapping intact exons; internal exon trapping using pSPL3 - protocols; 3-terminal exon trapping using pTAG4 - protocols; analysis and identification of trapped exons. Gene detection by the identification of cpG islands: comparison of the cpGislands and vertebrate genomic DNA; cpG islands as markers for genes; evolution of approaches for identifying CpG islands; recently developed techniques for identifying CpG islands; advantages of isolating CpG islands; advantages of isolating CpG islands as an approach to gene identification; choosing a strategy for isolating genes associated with CpG islands; combining approaches for gene isolation; using RCREs to identify CpG-rich sequences in cloned genomic DNA - protocols; data analysis for identification of genes associated with CpG islands from cloned genomic DNA - protocols; using a column that binds methylated DNA to purify CpG islands from uncloned genomics DNA - protocols. Detection of DNA variation: overview of DNA variation; methods for detecting DNA variation; choosing a suitable approach for mutation detection; the SSCP method; protocols for detecting DNA variation using SSCP analysis; the DGGE method; equipment and protocols for detecting DNA variation using DGGE: RNase cleavage at mismatches; protocols for detecting DNA variation usng Rnase cleavage; the CCM method; protocols for detecting DNA variation using CCM; direct sequencing of uncloned PCR products. (Part contents)


Best Sellers


Product Details
  • ISBN-13: 9780879695101
  • Publisher: Cold Spring Harbor Laboratory Press,U.S.
  • Publisher Imprint: Cold Spring Harbor Laboratory Press,U.S.
  • Height: 230 mm
  • Sub Title: Vol.2 A Laboratory Manual
  • ISBN-10: 0879695102
  • Publisher Date: 01 Dec 1997
  • Binding: Hardback
  • Series Title: v. 2 Genome Analysis Laboratory Manual S.


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)
Cold Spring Harbor Laboratory Press,U.S. -
Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Detecting Genes: Vol.2 A Laboratory Manual(v. 2 Genome Analysis Laboratory Manual S.)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    Fresh on the Shelf


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!