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Home > Mathematics and Science Textbooks > Biology, life sciences > Mycology, fungi > Methods in Yeast Genetics: A Laboratory Course Manual
Methods in Yeast Genetics: A Laboratory Course Manual

Methods in Yeast Genetics: A Laboratory Course Manual


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About the Book

This edition of the Cold Spring Harbor Laboratory Press's yeast genetics course manual incorporates elements from the 1994 and 1990 publications. It also contains information on additional techniques such as mitotic recombination and random spore analysis, cytoduction, karyogamy, telomeric silencing, transformation techniques, and isolation of genomic DNA.

Table of Contents:
Genetic nomenclature. Looking at yeast cells. Experiments: isolation and characterization of auxotrophic, temperature-sensitive, and UV-sensitive mutants; meiotic mapping; mitotic recombination and random spore analysis; transformation of yeast; cytoduction and karyogamy; gene replacement; isolation of ras2 suppressors; manipulating cell types; isolation of suppressors of telomeric silencing by insertional shuttle mutagenesis; lacZ gene fusion expression in yeast; immunofluorescent staining of yeast cells. Techniques and protocols: super-efficient transformation of yeast with lithium acetate/SS-DNA/PEG; other yeast transformation methods; yeast colony hybridization; yeast DNA isolations; yeast protein extracts; yeast RNA isolation; northern analysis - formaldehyde agarose gel, blotting, and hybridization to filters; alkaline southern blotting procedure; scoring killer factor; hydroxylamine mutagenesis of plasmid DNA; assay of galactosidase in yeast; transformation of bacteria (CaC12 method); plate assay for carboxypeptidase; western blotting protein extracts; random spore analysis; yeast vital strains; yeast immunofluorescence with antibodies; actin staining in fixed cells; PCR analysis of genotype; quick e coli plasmid DNA mini-prep. Appendices: media; stock preservation; yeast genetic map; grids; electrophoretic karotypes of strains for southern blot mapping; strains; counting yeast cells with a standard hemocytometer chamber.


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Product Details
  • ISBN-13: 9780879695088
  • Publisher: Cold Spring Harbor Laboratory Press,U.S.
  • Publisher Imprint: Cold Spring Harbor Laboratory Press,U.S.
  • Height: 230 mm
  • Returnable: N
  • ISBN-10: 0879695080
  • Publisher Date: 24 Nov 1997
  • Binding: Paperback
  • Language: English
  • Sub Title: A Laboratory Course Manual


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Methods in Yeast Genetics: A Laboratory Course Manual
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