Methods in Plant Biochemistry and Molecular Biology

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

Table of Contents:
Structure Methods for Analysis of Plant Cell/Tissue Ultrastructure, J.E. Mayfield and W.V. Dashek Carpogenesis and Basidiosporogenesis, S.C. Croan Decoloration of Wood Sapstain, S.C. Croan Chemistry Isolation, Assay, and Characterization of Plant Carbohydrates, W.V. Dashek Assay and Purification of Enzymes-Oxalate Decarboxylase, W.V. Dashek and J.A. Micales Antibody-Mediated Immunochemistry and Immunoassay in Plant Related Diseases, C. Clausen and F. Green, III Extraction and Assay of Plant Lipids: Phospholipids, W.V. Dashek Isolation and Analysis of Plant Nucleic Acids, W.V. Dashek Isolation, Separation, and Characterization of Organic Acids, W.V. Dashek and J.A. Micales Photoaffinity Labeling with 5-Azidoindole-3-Acetic Acid, A. Jones and T. Lomax Methods for the Analysis of Cytokinin Content, Metabolism, and Response, D.A. Lightfoot, K.L. McDaniel, J.K. Ellis, R.H. Hammerton, B. Nicander Analysis of Ethylene Biosynthesis in Plant Tissue by GC/FID, M.A. Harrison The Role of Plant Growth Regulators During Filament and Floral Development in Ipomoea nil Flowers, H. Kiss Extraction and Assay of Terpenoids-Including Certain Plant Hormones, W.V. Dashek Analytical Methods for the Analysis of Alkaloids, W.V. Dashek Phenolics and Compartmentalization in the Sapwood of Broad-Leaved Trees, K.T. Smith Lignin Analysis, J.F. Dean Flavanoid Applications in Research, W.D.. Clark and G. P. Titus Analysis and Manipulation of the Chorophyll Pathway in Higher Plants, M.V. Duke and S.O. Duke Isolation and Characterization of Plant and Algal Pigment-Protein Complexes, T.A. Martinson and F.G. Plumley The Isolation and Assay of Elicitins, L.M. Yu Chemistry, Extraction, and Assay of Plant Vitamins, W.V. Dashek Metabolism Simultaneous Measurement of Oxygen Uptake and Quinone Pool Reduction in Potato Tuber Mitochondria, N.R. Bowlby, C.B. Hiser, and L. McIntosh Biosynthesis of Plant Cell Wall Polysaccharides, W.V. Dashek Carbohydrolase Assays, T.L. Highley In Vitro Synthesis of Plant Proteins: Polyphenol Oxidase, W.V. Dashek Plant Lipid Protocols: Biochemical Analyses of Fatty Acid Metabolism in Higher Plants, D.J. Guerra and K. Dziewanowska cDNA Library Construction, W.V. Dashek Metabolism of Plant Hormones, W.V. Dashek Lignin Degradation, W.V. Dashek Plant Molecular Biology Plant Transformation Techniques and Vectors, W.S. Grayburn Restricted Fragment Length Polymorphism, W.V. Dashek Amplification of DNA and Gene Isolation, R. March-Amegadzie Molecular Analysis of cis -Acting Transcriptional Regulatory Elements and Transcriptional Factors, in the Bean Storage Protein Phaseolin Gene, N. Murai Manipulation of Plant Gene Expression Using Antisense RNA, D. Lee and C.J. Douglas Index NTI copy: Already written for August package Back Cover Copy/Short copy

Review :
"the manual will satisfy all who wish to make their library, knowledge, or both more complete in respect of modern methods of plant biochemistry and molecular biology." (Photosynthetica International Journal) "Flowcharts together with the format of the book and its spiral binding make the technique easy to follow with the book on the lab bench while performing an experiment. The chapters are well referenced.Its relatively low price should contribute to the wide diffusion this good book deserves. The book aimed at trained scientists will be an excellent tool that should be present in every plant biochemistry and physiology laboratory. The protocols are clear, novel, written by experimentators for experimentators." -Michel Couderchet, Reims