Methods in Cellular Immunology, Second Edition
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Methods in Cellular Immunology, Second Edition

Methods in Cellular Immunology, Second Edition


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About the Book

Methods in Cellular Immunology provides a guide to cellular immunological methods. This indispensible book covers both murine and human models. The described methods involve cell isolation and separation; antibody production, detection, and isolation; flow cytometry; NK cytotoxicity; and quantitation of cytokines and cytokine receptors.This practical, easy-to-follow methods book presents step-by-step descriptions of the most common techniques of cellular immunology in a style that both novices and seasoned scientists will be able to understand. Text includes the background information necessary to perform basic techniques. An extensive list of references is provided, allowing readers to compare the methods in the book with the original techniques. The book also lists the manufacturers of all chemicals and materials used in the procedures. Both scientists and researchers involved in basic and clinical research and all scientists wishing to employ immunological techniques will benefit from this valuable resource.

Table of Contents:
Cells Preparation of Cell Suspensions Isolation of Cells References Flow Cytometry Proliferation Assays Incorporation of Tritiated Thymidine into DNA Nonradioactive Detection Methods (MTT Assay) References Preparation of Cell Clones Preparation of Mouse T Cell Clones Preparation of Human T Cell Clones Preparation of Human B Cell Clone Cloning by Limiting Dilution Technique References Cytotoxic Cells Cell-Mediated Cytotoxicity References Assays of Cytokines Introduction Bioassays Immunoassays References Cytokine Receptors Preparation and Modification of Antibodies Polyclonal Antibodies Monoclonal Antibodies Coupling of Synthetic Peptides to Carrier Proteins References Detection of Antibodies Hemolytic Plaque Assays ELISA References Purification of Immunoglobulins Precipitation by Ammonium Sulfate Caprylic Acid-Ammonium Sulfate Isolation of IgG from Ascites Fluid Purification of Antibodies by Precipitation with Polyethylene Glycol Purification of Antibodies from Culture Supernatants by Precipitation with Polyethylene Glycol Affinity Chromatography Using Protein A-Sepharose Purification of IgM Ammonium Sulfate Precipitation of IgM Purification of Anti-Hapten Antibodies by Affinity Chromatography Fragmentation of Immunoglobulin into F(ab) Fragments using Papain Fragmentation of Immunoglobulin into F(ab) Fragments using Preactivated Papain Fragmentation of Immunoglobulin into F(ab)2 Fragments using Pepsin Double Immunodiffusion Assay References Buffers and Media Commercial Sources Index


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Product Details
  • ISBN-13: 9780849365485
  • Binding: Paperback
  • Language: English
  • No of Pages: 246
  • Weight: 372 gr
  • ISBN-10: 0849365481
  • Height: 235 mm
  • No of Pages: 246
  • Returnable: N
  • Width: 156 mm


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