Laboratory Methods in Immunology

This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.

Table of Contents:
VOLUME II: ANIMAL WORK, IN VIVO STUDIES. Basic Techniques with Experimental Animals. Immunological Adjuvants: General Properties, Advantages, and Limitations. Labeling of Lymphocytes for In Vivo Tracing. Photoimmunology: Ultraviolet Radiation Effects on the Immune System. ANTIGEN DETECTION IN TISSUES AND CELLS. Ultrastructural Demonstration of Cell Surface and Intracellular Class II Major Histocompatibility Antigens by Monoclonal Antibodies Using a Pre-Embedding Indirect Immunoperoxidasae Technique. A Microplate ELISA for the Detection of Internal and External Cellular Antigens. IMMUNOAFFINITY PURIFICATION. The Use of Immunoaffinity Chromatography in the Preparation of Therapeutic Products. Methodology for the Immunopurification and Immunodepletion of Coagulation Factors, with Emphasis on Factors VIII and IX. Improved Cyanogen Bromide Activation. A User Guide to Protein A. Immunoaffinity Adsorption: Practical Application in Laboratory Research and Production. Production and Characterization of Solid-Phase Immunoadsorbents. Index. Catalog no. 4482...Approx. U.S. $124.94/Outside U.S. $147.00.