Microbial Genome Methods

Learn to apply powerful molecular biology and genetics techniques to your microbial research with this easy-to-understand, practical guide. Microbial Genome Methods distills complex experimental procedures into fundamental techniques that can be readily adapted to your own research. In addition, this book provides background information specifically relevant to microbial research, as well as descriptions of expected results. The book is divided into two sections. Section one covers eukaryotic microbes, with emphasis on the yeast Saccharomyces cerevisiae. Section two covers prokaryotic microbes, focusing on Escherichia coli, and other important bacteria. These microbes were selected because they are widely studied and understood, but all information can be easily applied to the microbe you are investigating. Chapters are written by nationally and internationally recognized research scientists currently active in this field. Don't miss out on the tremendous boost these research techniques can give your work.

Table of Contents:
Eukaryotic Microbes Molecular Dissection of G Protein-Mediated Signal Transduction Using Random Mutagenesis in Dictyostelium, C.A. Parent and P.N. Devreotes Cloning and Characterizing Developmentally Regulated Genes in Candida albicans, D.R. Soll, T. Srikantha, and S.R. Lockhart Methods in the Analysis of Telomere Function in Saccharomyces cerevisiae, A.J. Lustig Generation, Screening, and Analysis of LACZ Fusions in Yeast, N. Burns, P. Ross-MacDonald, G.S. Roeder, and M. Snyder Development of a Genetic Selection for Transcription Factor Mutants Based on a Promoter Fusion to a G418 Resistance Gene in Saccharomyces cerevisiae, A.E. Pepper and M.J. Holland Generation of Chimeric Proteins by in Vivo Homologous Gene Recombination in Yeast: Example of an Essential P-Type ATPase, A. de Kerchove d'Exaerde, P. Supply, and A. Goffeau Comparative Gene Analysis and Dominant Negative Mutations in DNA Repair, G.T. Milne, J.W. Donovan, Y. Xiao, and D.T. Weaver DNA Repair and Checkpoint Controls in Fission Yeast: A Practical Guide, A.M. Carr and J.M. Murray Prokaryotic Microbes Techniques for the Introduction of Specific DNA Lesions Downstream from Prokaryotic RNA Polymerase Promoters and Analysis of the Transcription Products, W. Zhou and P.W. Doetsch Bacteria Identification by PCR-Based Techniques, G. Damiani, P. Amedeo, C. Bandi, R. Fani, D. Bellizzi, and V. Sgaramella Live Time Quantification of Bacterial Interactions in Various Environments, C.I. Kado Isolation of Complexes Containing an Escherichia coli Chaperone Protein, SecB, Bound to Newly Synthesized Polypeptides, O. Francetic and C.A. Kumamoto Pathogenesis and Host Resistance to Infection: A Model System and an Analysis of Capsule Synthesis and Regulation by Bacillus anthracis, S.L. Welkos and R. Marrero Genetic Manipulation of Bordetella, V. Scarlato, S. Ricci, R. Rappuoli, and M. Pizza