The Metabolic Pathway Engineering Handbook, Two Volume Set: (Metabolic Pathway Engineering Handbook)

Christina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. Fundamentals The first volume provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus subtilis, Streptomyces, filamentous fungi, annd mammalian cells using cell culture. Tool and Applications The second volume delves into evolutionary tools, including those associated with gene expression for metabolic pathway engineering. It covers applications of emerging technologies, including research on genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. It also evaluates emerging applications for producing fine chemicals, drugs, and alternative fuels.

Table of Contents:
Volume I Introduction and Preface to Handbook, Christina D. Smolke Progression of biological synthesis methods towards commercial relevance The field of metabolic engineering An overview of the metabolic pathway engineering handbook Metabolic engineering: looking towards the future Section I. Cellular Metabolism, Vincent Martin Transport processes inside the cell Energy generating reactions Biosynthesis of important building blocks Polymerization of building blocks to macromolecules Rare metabolic conversions – harvesting diversity through nature Section II. Balances and Reaction Models, Walter van Gulik Growth nutrients and diversity Mass balances, rates, and experiments Data reconciliation and error detection Black box models for growth and product formation Metabolic models for growth and product formation A thermodynamic description of microbial growth Section III. Regulation of Metabolic Pathways, James Liao Regulation of specific pathways Regulating of secondary metabolism Building networks as assemblies of simpler control schemes Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas Metabolic flux analysis Metabolic control analysis Structure and flux analysis of metabolic networks Constraint-based genome-scale models of cellular metabolism Multi-scale modeling of metabolic regulation Validation of metabolic models Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen Escherichia coli as a well-developed host for metabolic engineering Metabolic engineering in yeast Metabolic engineering in Bacillus subtilis Metabolic engineering in Streptomyces Metabolic engineering in filamentous fungi Metabolic engineering in mammalian cells using cell culture   Volume II Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert Evolutionary engineering of industrially important microbial phenotypes Expanding enzyme function and activity through molecular evolution Evolving the function of DNA and RNA regulatory regions through molecular evolution Evolving pathways and genomes for the production of natural and novel compounds Models predicting optimized evolutionary strategies Section VII. Gene Expression Tools for Metabolic Pathway Engineering, Christina Smolke Low-copy number plasmids as artificial chromosomes Chromosomal engineering strategies Regulating gene expression through engineered RNA technologies Tools designed to regulate translational efficiency Regulating gene expression through post-translational modifications Engineering bifunctional enzymes for optimized metabolite transfer between sequential conversion steps Practical pathway engineering – demonstration in integrating tools Section VIII. Applications of Emerging Technologies to Metabolic Engineering, Jay Keasling Genome-wide technologies: DNA and phenotypic microarrays, proteomics Monitoring and measuring the metabolome Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee Genome-scale models, systems biology, and metabolic engineering Cell-free systems for metabolic engineering Dynamic in silico cell models Appendix I: Tools for Experimentally Determining Flux through Pathways, Ralf Takors Measuring flux by isotope labeling Tools for measuring intermediate and product formation Quantitative analysis of labeling data Appendix II: Applications, Brian Pfleger and Brian Brazeau Cofactor regulation in biocatalytic redox reactions Metabolic engineering for fine chemical production Metabolic engineering for drug production Metabolic engineering for alternative fuels  

About the Author :
Stanford University, California, USA