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Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition

Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition


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About the Book

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era.Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study.H andbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniquesNew chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry librariesWith this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Table of Contents:
Isolation and Purification of DNA Isolation and Purification of RNA Extraction and Purification of Proteins Preparation of Nucleic Acid Probes Southern Blot Hybridization Northern Blot Hybridization Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s) Western Blot Hybridization cDNA Libraries Genomic DNA Libraries DNA Sequencing Analysis DNA Mutagenesis DNA Footprinting and Gel Retardation Assay Differential Display Functional Genomics and DNA Microarray Technology Translation of mRNA(s) and Analysis of Proteins by Gel Electrophoresis Plant Tissue and Cell Culture Gene Transfer and Expression in Animals Gene Transfer and Expression in Plants Inhibition of Gene Expression Light, Scanning Electron, Environmental Scanning Electron, Transmission Electron, and Confocal Microscopy Localization of Gene Expression Bioseparation Techniques and Their Applications Combinatorial Techniques Computational Data Mining Methods Applied to Combinatorial Chemistry Libraries Subject Index


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Product Details
  • ISBN-13: 9780849308154
  • Publisher: Taylor & Francis Inc
  • Publisher Imprint: CRC Press Inc
  • Edition: New edition
  • Language: English
  • No of Pages: 600
  • Weight: 1052 gr
  • ISBN-10: 0849308151
  • Publisher Date: 24 Nov 2003
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 600
  • Returnable: N
  • Width: 178 mm


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