Chromatographic Methods
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Chromatographic Methods

Chromatographic Methods


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About the Book

This work presents an introduction to chromatography and reflects the significant developments since the mid-1980s, particularly in instrumentation and column technology, and control and data processing. The chapters on chromatographic theory, GC, HPLC, hybrid techniques, and data processing have been expanded in this edition to give a more detailed coverage while newer techniques such as capillary elctrophoresis are also presented. The chapter on practical experiments has also been revised and features a number of new exercises in GC, GCMS, HPLC with diode array detection, and capillary elctrophoresis. This text is aimed at undergraduate and postgraduate students, as well as R&D personnel in academic and industrial laboratories allowing them to develop methods and establish optimum separation procedures.

Table of Contents:
1 Introduction.- 1.1 Introduction to chromatography.- 1.2 History of chromatography.- 1.3 Classification of chromatographic methods.- References.- 2 Theoretical considerations.- 2.1 Introduction.- 2.2 Factors influencing retention.- 2.3 Retention and equilibrium in chromatography.- 2.4 Separating efficiency of a column.- 2.5 Band broadening processes.- 2.6 Resolution.- 2.7 Quantification in chromatography.- References.- 3 Planar chromatography.- 3.1 Thin layer chromatography.- 3.2 Paper chromatography.- 3.3 Electrophoresis.- 3.4 Capillary electrophoresis.- References.- 4 Liquid phase chromatography on open columns.- 4.1 Introduction.- 4.2 Practical aspects and considerations.- 4.3 Modes of chromatography.- 4.4 Adsorption chromatography.- 4.5 Partition column chromatography.- 4.6 Ion exchange chromatography.- 4.7 Inorganic ion exchangers.- 4.8 Gel ion exchangers.- 4.9 Gel chromatography.- 4.10 Affinity chromatography.- 4.11 Covalent chromatography.- 4.12 Chromatofocusing.- 4.13 Flash chromatography.- References.- 5 Gas chromatography.- 5.1 Introduction.- 5.2 Principles of gas chromatography.- 5.3 Resolution.- 5.4 Columns and stationary phase.- 5.5 Choice of stationary phase.- 5.6 Capillary column gas chromatography.- 5.7 Gas-solid chromatography.- 5.8 Gas chromatography instrumentation.- 5.9 Sampling techniques.- 5.10 Detectors for gas chromatography.- 5.11 Supercritical gas chromatography.- 5.12 Applications of gas chromatography.- References.- 6 High performance liquid chromatography.- 6.1 Introduction.- 6.2 Modes of chromatography.- 6.3 Overview of high performance liquid chromatography instrumentation.- 6.4 Theory.- 6.5 Detailed discussion of high performance liquid chromatography instrumentation.- 6.6 Column packings and stationary phases for liquid chromatography.-6.7 Adsorption chromatography.- 6.8 Liquid—liquid partition chromatography.- 6.9 Chemically bonded stationary phases for high performance liquid chromatography.- 6.10 Chiral chromatography.- 6.11 Ion exchange chromatography.- 6.12 Ion pairing.- 6.13 Size exclusion chromatography.- 6.14 Liquid chromatography method development.- 6.15 Quantitative analysis.- 6.16 Preparative liquid chromatography.- 6.17 Microcolumns in liquid chromatography.- 6.18 Applications.- References.- 7 Chromatography and spectroscopic techniques.- 7.1 Introduction.- 7.2 Chromatographic requirements.- 7.3 Chromatographic and mass spectrometry techniques.- 7.4 Gas chromatography interfacing techniques.- 7.5 High performance liquid chromatography interfacing.- 7.6 Chromatography and mass spectrometry data systems.- 7.7 Infrared spectrophotometry.- 7.8 Ultraviolet—visible spectrophotometry.- 7.9 Atomic spectroscopy.- References.- 8 Processing chromatographic data.- 8.1 Introduction.- 8.2 The chromatogram.- 8.3 Data collection and processing.- 8.4 Chromatography software.- 8.5 Method development and optimisation.- 8.6 Laboratory information management systems.- References.- 9 Model or practical experiments in chromatographic techniques.- 9.1 Introduction.- 9.2 List of experiments.- 9.3 Section A. Paper chromatography.- 9.4 Section B. Electrophoresis and related techniques.- 9.5 Section C. Thin layer chromatography.- 9.6 Section D. Column chromatography.- 9.7 Section E. Gas chromatography.- 9.8 Section F. High performance liquid chromatography.- 9.9 Section G. Capillary electrophoresis.- References.- 10 Illustrative problems in chromatography.- 10.1 Introduction.- 10.2 Plate theory and resolution.- 10.3 Van Deemter.- 10.4 Volatility and temperature in gas chromatography.- 10.5 Qualitativeanalysis.- 10.6 Quantitative analysis.- 10.7 Composite questions.- Appendix 1 Glossary of chromatographic terms.- Appendix 2 Table of chromatography symbols.

Review :
comprehensive and well presented...The chapters are informative and easy ot read...already used widely in teaching institutions and this use will undoubtedly continue in the future because it provides an ideal resource for this purpose. I commend it highly - Journal of Chromatography


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Product Details
  • ISBN-13: 9780751401585
  • Publisher: Chapman and Hall
  • Publisher Imprint: Chapman and Hall
  • Edition: Revised edition
  • Language: English
  • Returnable: N
  • ISBN-10: 0751401587
  • Publisher Date: 31 Dec 1995
  • Binding: Paperback
  • Height: 235 mm
  • No of Pages: 580
  • Width: 155 mm


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