A Practical Guide to Molecular Cloning
Home > Mathematics and Science Textbooks > Biology, life sciences > Molecular biology > A Practical Guide to Molecular Cloning
A Practical Guide to Molecular Cloning

A Practical Guide to Molecular Cloning


     0     
5
4
3
2
1



Out of Stock


Notify me when this book is in stock
X
About the Book

This new edition of the standard laboratory manual is intended to make molecular cloning faster and easier. It presents techniques tested at the Curie Institute and at other leading laboratories, and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. A detailed explanation of protocols is offered, allowing the isolation, cloning, and expression of genes from living species. As well as presenting up-to-date techniques on sequencing, in vitro expression of cloned gene and use of computers for study of nucleic acids, it shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes.

Table of Contents:
Laboratory Equipment Needed for Molecular Cloning; A Few Words about Safety; Basic Methods in Molecular Biology; Useful Modifying Enzymes for Molecular Cloning; Restriction Endonucleases; Vectors for Molecular Cloning; Purification and Characterization of Vector and Passenger DNA; Digestion of DNA with Restriction Endonucleases; Separation of DNA Fragments by Electrophoresis; Ordering the Restriction DNA Fragments in Physical Maps; Purification of DNA Fragments; Modification of DNA Fragments with Cohesive Termini; Ligation; Propagation of Recombinant DNA Molecules; Characterization of Recombinant Clones; Preparation of Genomic Libraries; Purification and Characterization of RNA Species; Cloning of cDNA Species; Sequencing of DNA; RNA Sequencing; Use of Computerized Programs in the Treatment of DNA Sequence Information; Localized Mutagenesis; Methods for Studying DNA-Protein Interactions; Expression of Cloned DNA Sequences In Vitro or In Procaryotic and Eucaryotic Cells; Epilogue: If You Want AVA I to Meet BAM Hi; Appendixes.


Best Sellers


Product Details
  • ISBN-13: 9780471850717
  • Publisher: John Wiley and Sons Ltd
  • Publisher Imprint: John Wiley & Sons Inc
  • Edition: Revised edition
  • Language: English
  • Weight: 2438 gr
  • ISBN-10: 0471850713
  • Publisher Date: 13 May 1988
  • Binding: Hardback
  • Height: 85 mm
  • Returnable: N
  • Width: 22 mm


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
A Practical Guide to Molecular Cloning
John Wiley and Sons Ltd -
A Practical Guide to Molecular Cloning
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

A Practical Guide to Molecular Cloning

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!