Primary and Stem Cells
Home > Science, Technology & Agriculture > Biochemical engineering > Biotechnology > Genetic engineering > Primary and Stem Cells: Gene Transfer Technologies and Applications
Primary and Stem Cells: Gene Transfer Technologies and Applications

Primary and Stem Cells: Gene Transfer Technologies and Applications


     0     
5
4
3
2
1



Available


X
About the Book

This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.

Table of Contents:
Foreword Michele Calos vii Preface ix Contributors xi Part I: Cloning and Gene Delivery 1 1. DNA Assembly Technologies Based on Homologous Recombination 3 Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen 2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19 Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut 3. Strategies for the Delivery of Naked DNA 37 Jennifer C. Moore and Ronald P. Hart Part II: Nonintegrating Technologies 49 4. Episomal Vectors 51 Junying Yu 5. Nonintegrating DNA Virus 85 Chris Kemp and David Kuninger 6. Nonintegrating RNA Viruses 103 Noemi Fusaki 7. Protein Delivery 119 Xiao-Jian Han and Kazuhito Tomizawa Part III: Integrating Technologies 135 8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137 Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor 9. Integrating Viral Vectors for Gene Modifications 163 Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek 10. Bacteriophage Integrases for Site-Specific Integration 199 Bhaskar Thyagarajan 11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211 Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu Part IV: Applications 227 12. Modified Stem Cells as Disease Models and in Toxicology Screening 229 Catharina Ellerstr€om, Peter Sartipy, Raimund Strehl, and Johan Hyllner 13. Screening and Drug Discovery 251 Elizabeth A. Davenport and Robert S. Ames Index 273

About the Author :
UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications. BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.

Review :
“This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells.  Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy.”  (Doody’s, 17 August 2012)      


Best Sellers


Product Details
  • ISBN-13: 9780470610749
  • Publisher: John Wiley & Sons Inc
  • Publisher Imprint: John Wiley & Sons Inc
  • Height: 239 mm
  • No of Pages: 312
  • Returnable: N
  • Sub Title: Gene Transfer Technologies and Applications
  • Width: 163 mm
  • ISBN-10: 0470610743
  • Publisher Date: 23 Dec 2011
  • Binding: Hardback
  • Language: English
  • Returnable: N
  • Spine Width: 20 mm
  • Weight: 608 gr


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Primary and Stem Cells: Gene Transfer Technologies and Applications
John Wiley & Sons Inc -
Primary and Stem Cells: Gene Transfer Technologies and Applications
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Primary and Stem Cells: Gene Transfer Technologies and Applications

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!