Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation
Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation
Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation

Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation

|
     0     
5
4
3
2
1




Out of Stock


Notify me when this book is in stock
About the Book

This text offers the first systematic compilation of various techniques and methodologies used in the analysis of intrinsically disordered proteins (IDPs). Its coverage addresses one of the hottest yet least documented areas in protein biophysics today, summarizing recent achievements in the methods for structural characterization of intrinsically disordered proteins. The text also addresses structural biology and protein degradation, along with a myriad of spectroscopic techniques that can be used to elucidate protein structure: NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis.

Table of Contents:
PREFACE. INTRODUCTION TO THE WILEY SERIES ON PROTEIN AND PEPTIDE SCIENCE. LIST OF CONTRIBUTORS. LIST OF ABBREVIATIONS. PART I ASSESSING IDPs IN THE LIVING CELL. 1 IDPs and Protein Degradation in The Cell ( Yosef Shaul, Peter Tsvetkov, and Nina Reuven ). 2 The Structural Biology of IDPs Inside Cells ( Philipp Selenko ). PART II SPECTROSCOPIC TECHNIQUES. 3 Nuclear Magnetic Resonance Spectroscopy Applied to (Intrinsically) Disordered Proteins ( Frans A. A. Mulder, Martin Lundqvist, and Ruud M. Scheek ). 4 Atomic-Level Characterization of Disordered Protein Ensembles Using NMR Residual Dipolar Couplings ( Martin Blackledge, Pau Bernado, and Malene Ringkjobing Jensen ). 5 Determining Structural Ensembles for Intrinsically Disordered Proteins ( Gary W. Daughdrill ). 6 Site-Directed Spin Labeling EPR Spectroscopy ( Valerie Belle,, Sabrina Rouger, Stephanie Costanzo, Sonia Longhi, and Andre Fournel ). 7 The Structure of Unfolded Peptides and Proteins Explored by Vibrational Spectroscopy ( Reinhard Schweitzer-Stenner, Thomas J. Measey, Andrew M. Hagarman, and Isabelle C. Dragomir ). 8 Intrinsically Disordered Proteins and Induced Folding Studied by Fourier Transform Infrared Spectroscopy ( Antonino Natalello and Silvia Maria Doglia ). 9 Genetically Engineered Polypeptides as a Model of Intrinsically Disordered Fibrillogenic Proteins: Deep UV Resonance Raman Spectroscopic Study ( Natalya I. Topilina, Vitali Sikirzhytski, Seiichiro Higashiya, Vladimir V. Ermolenkov, John T. Welch, and Igor K. Lednev ). 10 Circular Dichroism of Intrinsically Disordered Proteins ( Robert W. Woody ). 11 Fluorescence Spectroscopy of Intrinsically Disordered Proteins ( Eugene A. Permyakov and Vladimir N. Uversky ). 12 Hydration of Intrinsically Disordered Proteins From Wide-Line NMR ( Kalman Tompa, Monika Bokor, and Peter Tompa ). PART III SINGLE-MOLECULE TECHNIQUES. 13 Single-Molecule Spectroscopy of Unfolded Proteins ( Benjamin Schuler ). 14 Monitoring the Conformational Equilibria of Monomeric Intrinsically Disordered Proteins by Single-Molecule Force Spectroscopy ( Massimo Sandal, Marco Brucale, and Bruno Samori ). PART IV METHODS TO ASSESS PROTEIN SIZE AND SHAPE. 15 Analytical Ultracentrifugation, a Useful Tool to Probe Intrinsically Disordered Proteins ( Florence Manon and Christine Ebel ). 16 Structural Insights into Intrinsically Disordered Proteins by Small-Angle X-Ray Scattering ( Pau Bernado and Dmitri I. Svergun ). 17 Dynamic and Static Light Scattering ( Klaus Gast ). 18 Analyzing Intrinsically Disordered Proteins by Size Exclusion Chromatography ( Vladimir N. Uversky ). PART V CONFORMATIONAL STABILITY 545 19 Conformational Behavior of Intrinsically Disordered Proteins: Effects of Strong Denaturants, Temperature, PH, Counterions, and Macromolecular Crowding ( Vladimir N. Uversky ). 20 Detecting Disordered Regions in Proteins by Limited Proteolysis ( Angelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore, Erica Frare, and Marcello Zambonin ). PART VI MASS SPECTROMETRY. 21 Mass Spectrometry Tools for the Investigation of Structural Disorder and Conformational Transitions in Proteins ( Maria Samalikova, Carlo Santambrogio, and Rita Grandori ). PART VII EXPRESSION AND PURIFICATION OF IDPS. 22 Recombinant Production of Intrinsically Disordered Proteins for Biophysical and Structural Characterization ( Dmitri Tolkatchev, Josee Plamondon, Richard Gingras, Zhengding Su, and Feng Ni ). 23 Large-Scale Identifi cation of Intrinsically Disordered Proteins ( Vladimir N. Uversky, Marc S. Cortese, Peter Tompa, Veronika Csizmok, and A. Keith Dunker ). 24 Purification of Intrinsically Disordered Proteins ( Aviv Paz, Tzviya Zeev-Ben-Mordehai, Joel L. Sussman, and Israel Silman ). INDEX.


Best Sellers


Product Details
  • ISBN-13: 9780470602614
  • Publisher: John Wiley and Sons Ltd
  • Publisher Imprint: Wiley-Blackwell
  • Language: English
  • ISBN-10: 0470602619
  • Publisher Date: 09 Sep 2010
  • Binding: Other digital
  • No of Pages: 736


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation
John Wiley and Sons Ltd -
Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals

    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!