Neuroscience Protocols: Module 4

"Neuroscience Protocols" (NP) is a laboratory manual which provides protocols of new and established techniques used in neuroscience. The protocols are supplied in a loose-leaf format, with a ring binder. This makes it easy to remove the protocol you are using, or replace it with an update. NP is a compilation of modules. Each module contains about 10-12 new or updated protocols covering several subjects. Module 1 was published in May 1993; Module 2 in December 1993. Initially two modules will be published each year. Each new module contains updated cumulative contents of modules already published. The contents of the modules are available on request. A ringbinder with sectional dividers is part of the Module 1 and 2 set and is also available separately. NP is divided into the following sections: Behavioural Neurobiology, Cellular and Molecular Neurobiology, Developmental Biology, Neuroimmunology, Neuromorphology, Neuropharmacology and Neurochemistry, Neurophysiology, and Quantitative Neurobiology. The first NS CD-ROM is scheduled for publication in 1995. The electronic version will include most of the protocols published so far. There will be a single-user version and a network version.

Table of Contents:
Part 1 Section 010 - behavioural neurobiology: the behavioural adaptation of the collision test - inference of axonal linkage and estimation of conduction velocity from effects of paired-pulse stimulation, P. Shizgal and B. Murray. Part 2 Section 020 - cellular and molecular neurobiology: analysis of neuronal mRNA levels by quantitative reverse transcriptase-polymerase chain reaction, B.J. Baumgartner and E.M. Barnes. Part 3 Section 030 - developmental neurobiology: organ culture - an in vitro system for the sustained growth of neonatal olfactory bulb, V.C. Russo et al; combining biotinylated dextran amine neuronal labeling and lacZ reporter gene labeling to study the relationship between identified neuronal populations and gene expression in the embryonic mouse nervous system, H.N. Nichols et al; slice cultures as a tool to study neuronal development and the formation of specific connections, B. Heimrich and M. Frotscher. Part 4 Section 050 - neuromorphology: dual anterograde axonal tracing with phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), J.L. Lanciego and F.G. Wouterlood; the Sihler stain - a technique for studying peripheral nerves in whole mount specimens, B.L. Wu and I. Sanders. Part 5 Section 060 - neuropharmacology and neurochemistry: measurement of pentose phosphate pathway activity by microdialysis in vivo and in a single incubation in vitro, O. Ben-Yoseph et al. Part 6 Section 070 - neurophysiology: study on superbond adhesion to the skull for chronic electrode implantation in the rat, I. Limoge-Lendais et al; multisite recordings of the brain in the in vivo rat using a voltage-sensitive dye, P. Litaudon and M. Cattarelli; the combined single cell recording - intracerebral microdialysis method in freely behaving rats, N. Ludvig et al; multichannel near-infrared optical imaging of brain activity, Y. Hoshi and M. Tamura. Part 7 Section 080 - quantitative neurobiology: estimation of the number and size of GABA-containing neurons in the cerebral cortex, C. Beaulieu.