About the Book
Immuno-oncology and Immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices, Immunofluorescent microscopy to quantify and characterize chromosomal instability, Spatial techniques for understanding the tumor microenvironment, Very high-flex immunophenotyping using co-linear cytofluorometric evaluation, Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology, Assessment of cytoplasmic dsDNA for cellular immunogenicty, and much more.
Additional sections cover Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example, Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua, Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients, Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization, and many other topics that will be of interest to readers.
Table of Contents:
1. In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices
Claudia Burrello, Karin E. de Visser and Tom Langelaar
2. Immunofluorescent microscopy to quantify and characterize chromosomal instability
Pippa F. Cosper, Kathryn Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki Messick, Ava Bryan and Sophie Bice
3. Spatial techniques for understanding the tumour microenvironment
Florent Ginhoux, Margaux GARDET and Kevin Mulder
4. Very high-flex immunophenotyping using co-linear cytofluorometric evaluation
Benjamin Izar
5. Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology
Benjamin Izar
6. Assessment of cytoplasmic dsDNA for cellular immunogenicty
Rongze Olivia Lu, Raymond Sun, Oishika Das and Isha Mondol
7. Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example
Iole Macchia Sr., Francesca Urbani and Valentina La Sorsa
8. Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua
Alexander J. Muller and Dema Alghabban
9. Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients
Paola Nistico, Maria Rosado, Giuseppe Frisullo, Giulia Campo and Miriam Petrella
10. Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization
Paola Nistico, Annalisa Tocci, Nicla Porciello, Belinda Palermo, Lorenzo Valenti, Anna Di Carlo and Francesca Di Modugno
11. Lymphocyte migration/invasion assays
Louis M. Weiner, Rachael Maynard and Alex Lekan
12. Flow-cytometry-based assay to study CD8+ T cell cytotoxicity against tumor cells.
Roberta Zappasodi, Alexey V Sarapulov and Marc-Anthony Rodriguez
13. High dimensional flow cytometry for conventional and unconventional T cell populations
Maria Paula Roberti, Andrea Leufgen, Conrad Rauber, Annika Steitz, Paula Daza and Maria Paz Gavilan
14. Optimized Phospho-Flow Cytometry for Quantitative Analysis of Early TCR-Proximal Signaling: Implications for T Cell Function in Onco-Immunology
Paola Nistico, Nicla Porciello, Annalisa Tocci, Giulia Campo and Belinda Palermo
About the Author :
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models. Norma received her master’s degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on “Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
