The Immunological Synapse Part C
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The Immunological Synapse Part C: Volume 193(Volume 193 Methods in Cell Biology)

The Immunological Synapse Part C: Volume 193(Volume 193 Methods in Cell Biology)


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About the Book

The Immunological Synapse - Part C, Volume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand γδ T cells and evaluate their cytotoxicity.

Table of Contents:
Preface Clément Thomas and Lorenzo Galluzzi 1. Analysis of immune synapse by STED imaging and 3D quantitative colocalization of lytic granule markers Claudio Giraudo 2. Imaging the immune synapse: three-dimensional analysis of the immune synapse Manuel Izquierdo 3. Image processing approaches for microtubule remodeling quantification at the immunological synapse Andres Alcover, Marie Juzans, Celine Cuche and Vincenzo Di-Bartolo 4. Isolation and characterization of primary NK cells and their KIR2DL1+ population enrichment Mira Barda-Saad 5. Chapter title to be confirmed Johannes B. Huppa and Janett Göhring 6. Flow Cytometry Conjugate Formation Assay between Natural Killer (NK) Cells and Their Target Cells Jacques Zimmer 7. Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors Katelyn Spillane, Anna Bajur and Hannah McArthur 8. Chapter title to be confirmed Rose Thierry 9. Quantitative analysis of the B Cell immune synapse formation using imaging techniques Isabel Yuseff 10. chapter title yet to confirmed Rose Thierry 11. Expansion of γδ T cells and their use in in vitro cytotoxicity assays Maria Alejandra Parigiani 12. A Comprehensive Guide to Studying the Immunological Synapse Using Imaging Flow Cytometry Céline Hoffmann, Clément Thomas, Liza Filali, Andrea Michela Biolato and Max Krecke

About the Author :
Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.


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Product Details
  • ISBN-13: 9780443218682
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Height: 235 mm
  • No of Pages: 280
  • Sub Title: Volume 193
  • Width: 191 mm
  • ISBN-10: 0443218684
  • Publisher Date: 04 Feb 2025
  • Binding: Hardback
  • Language: English
  • Series Title: Volume 193 Methods in Cell Biology
  • Weight: 580 gr


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The Immunological Synapse Part C: Volume 193(Volume 193 Methods in Cell Biology)
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