LIPOSOMES in GENE DELIVERY

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.

Table of Contents:
Introduction Gene Therapy Strategies in Gene Delivery Disease Applications of Gene Therapy Genetic Diseases Cancer Infectious Diseases Neurological Diseases Cardiovascular Diseases Other Diseases Nucleic Acids DNA Structure and Conformation DNA Stability and Interactions Theoretical Description of DNA DNA Condensation Recombinant DNA Technology Gene Expression Promoters and Enhancers Introns and PolyA Tails Complementary DNA Cytoplasmic Gene Expression Reporter Genes Vectors Gene Delivery Naked DNA Viral Systems Nonviral Delivery Systems Liposomes Lipids and Liposome Composition Cationic Lipids Electrostatic Properties of Charged Lipids and Liposomes Cationic Liposomes Liposome Preparation Methods Mechanism of Vesicle Formation Liposome Stability Characterization and Quality Control Interactions of Liposomes with Cells and In Vivo Toxicity or Safety of Cationic Liposomes Liposomes and Cell Membranes Liposome Applications Genosomes (DNA-Lipid Complexes) Reactivity and Stability Structure of Cationic Lipid-DNA Complexes Characterization Pharmacokinetics and Biodistribution Toxicity Genosomes for Gene Delivery and Transfection Expression In Vitro Gene Transfection and Expression In Vivo Parenteral Applications of Genosomes Pulmonary Delivery Other Delivery Routes Genosome Administration into Humans Structure-Activity Relationships Structure-Activity Relationships of Cationic Lipids on the Molecular Level Structure-Activity Relationships on the Colloidal Level Mechanism of Transfection Transfection In Vitro Transfection In Vivo Factors and Steps That Determine Transfection Other Carrier Systems and Artificial Virus Polyelectrolytes Combination Complexes Liposomes in the Delivery of Antisense Oligonucleotides Introduction Antisense Oligonucleotides: Structure and Mechanism of Action Liposomes for the Delivery of Antisense Oligonucleotides An Overview of Some of the Results Ribozymes Delivery of Proteins Perspectives and Conclusion Cationic Liposomes Anionic Liposomes Sterically Stabilized Liposomes Conclusion and Afterword References Appendix A: Some Fundamental Concepts Appendix B: Periodic System of Elements Appendix C: Short Tutorial on Different Units Appendix D: Geometric Parameters of Liposomes and DNA Appendix E: Abbreviations Appendix F: Glossary Index

About the Author :
Danilo D. Lasic