Correlative Light and Electron Microscopy V
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Home > Mathematics and Science Textbooks > Biology, life sciences > Cellular biology (cytology) > Correlative Light and Electron Microscopy V: Volume 187(Volume 187 Methods in Cell Biology)
Correlative Light and Electron Microscopy V: Volume 187(Volume 187 Methods in Cell Biology)

Correlative Light and Electron Microscopy V: Volume 187(Volume 187 Methods in Cell Biology)


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About the Book

Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

Table of Contents:
1. How to Apply the Broad Toolbox of Correlative Light and Electron Microscopy to Address a Specific Biological Question Thomas Müller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth 2. Some tips and tricks for a correlative light electron microscopy workflow using stable expression of fluorescent proteins Paul Verkade 3. Targeting membrane receptors with fluoronanogold probes for high resolution correlative microscopy. Monica Fernandez Monreal 4. Correlative light and electron microscopy at defined cell cycle stages in a controlled environment Shotaro Otsuka 5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy Paul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew Davidson 6. Tissue CLEM Christine Longin 7. Array tomography of in vivo labeled synaptic receptors Christian Stigloher and Sebastian Britz 8. Correlative cryo-microscopy pipelines for in situ cellular studies Anna Sartori and Chiara Zurzolo 9. Building a super-resolution fluorescence cryomicroscope Thom Sharp 10. Analysis of super resolution CLEM data Thom Sharp 11. Nanometer targeting using cryo super resolution Laura Carolina Zanetti-Domingues 12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and Cryo-Fluorescence Microscopy Kenneth Fahy

About the Author :
Thomas Müller-Reichert works in the Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.


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Product Details
  • ISBN-13: 9780323951418
  • Publisher: Elsevier Science & Technology
  • Publisher Imprint: Academic Press Inc
  • Height: 229 mm
  • No of Pages: 336
  • Sub Title: Volume 187
  • Width: 152 mm
  • ISBN-10: 0323951414
  • Publisher Date: 30 Apr 2024
  • Binding: Hardback
  • Language: English
  • Series Title: Volume 187 Methods in Cell Biology
  • Weight: 670 gr


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Correlative Light and Electron Microscopy V: Volume 187(Volume 187 Methods in Cell Biology)
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