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Laboratory Directions for Beginners in Bacteriology; An Introduction to Practical Bacteriology for Students and Practitioners of Comparative and of Human Medicine

Laboratory Directions for Beginners in Bacteriology; An Introduction to Practical Bacteriology for Students and Practitioners of Comparative and of Human Medicine


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Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: Ion is spread upon a properly cleansed cover-glass it will not roll up in droplets, but remain in a thin, even layer on the surface.) Treat the slides with the cleaning mixture in the same manner as the cover-glasses. They may, however, be wiped directly out of the rinsing water. Slides can often be cleaned very satisfactorily by washing them in hot soap-suds, rinsing them in water, and wiping them with a soft cloth. (f) Cleaning used culture apparatus. Place the tubes, flasks, or Petri dishes containing old cultures in a water bath, cover them with water, add a little sal soda (about an ounce to the gallon of water), and boil for 20 minutes. Pour off the water and empty the tubes, after which again boil them for 5 minutes in clean soap and water. Then wash and treat with the cleaning mixture the same as the new tubes. Cultures of spore-bearing pathogenic bacteria, such as those of anthrax, should be destroyed by heating in the autoclave at a temperature of at least 110 C. for half an hour before the tubes are emptied and washed. 4. A Method fpr Cleaning Cover-glasses for Flagella Stain. ? For this work the ordinary method of cleaning cover-glasses is not sufficient, although the heating will often give a perfectly satisfactory cover-glass. The following treatment was highly recommended to me by Dr. Erwin F. Smith. First clean the cover-glasses by the ordinary method, after which boil them in an agate cup or glass beaker in a 10% solution of caustic soda for 5 minutes. After cooling, rinse the cover-glasses thoroughly in distilled water, place them in a beaker and cover them with a i % solution of hydrochloric acid, heat to the boiling point, and allow to stand for several hours (over night or longer). Then pour the acid off and rinse the cover- glasses several times in d...


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Product Details
  • ISBN-13: 9780217784795
  • Publisher: General Books
  • Publisher Imprint: General Books
  • Height: 246 mm
  • No of Pages: 38
  • Spine Width: 2 mm
  • Width: 189 mm
  • ISBN-10: 0217784798
  • Publisher Date: 15 Jan 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 86 gr


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Laboratory Directions for Beginners in Bacteriology; An Introduction to Practical Bacteriology for Students and Practitioners of Comparative and of Human Medicine
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