The Technology of Bacteria Investigation; Explicit Directions for the Study of Bacteria Their Culture, Staining, Mounting, Etc
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The Technology of Bacteria Investigation; Explicit Directions for the Study of Bacteria Their Culture, Staining, Mounting, Etc

The Technology of Bacteria Investigation; Explicit Directions for the Study of Bacteria Their Culture, Staining, Mounting, Etc


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About the Book

Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: protected from all dust. Precautions. Not only the plates, the bell-glass and the clockwork need to be carefully coated with vaseline with a brush, but it is necessary to cover the mercury with a thin layer of aseptic glycerine. Moreover the air of the two bell-glasses must lie kept constantly saturated with moisture; this may be easily accomplished by placing in a little glass dish under each cylinder a sponge soaked with a saturated sublimate solution. Hesse's Method for the quantitative estimation of micro-organisms contained in the air is to draw the air through long glass tubes whose walls are coated with stiffened Koch's culture gelatine. The air stream is regulated by means of an aspirator, and at the same time measured. The number of the colonies springing up in the gelatine, and the quantity of air aspirated, give approximate figures for the quantity of germs in the air. Hesse's apparatus consists of a glass tube (as a rule 90 cm. long and 3.5 cm. in diameter), closed at one end by two rubber caps, the inside one having a round hole about 1 cm. in diameter cut in the centre. The other end has a perforated rubber plug 2 cm. thick, through which passes a glass tube with cotton in both ends. The apparatus is sterilized by steam at 100 C. for two hours, and is then coated inside with culture gelatine, which is spread evenly by proper movement, the bulk being allowed to settleon the lower side, the tube being held horizontally. It is allowed to cool, and the tube placed on a tripod such as is used for photography, and the small glass tube passing through the stopper is connected, by means of a rubber tube, with an aspirator made with two one-litre flasks connected by rubber tubes, and hung to the same stand, the upper one filled with water acting as an aspirator. When all ...


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Product Details
  • ISBN-13: 9780217374217
  • Publisher: General Books
  • Publisher Imprint: General Books
  • Height: 246 mm
  • No of Pages: 56
  • Spine Width: 3 mm
  • Width: 189 mm
  • ISBN-10: 0217374212
  • Publisher Date: 12 Jan 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 118 gr

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The Technology of Bacteria Investigation; Explicit Directions for the Study of Bacteria Their Culture, Staining, Mounting, Etc
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