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A Laboratory Course in Serum Study

A Laboratory Course in Serum Study


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Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: LESSON XX WASSERMANN REACTIONS Technique Of Tests I. Preliminary Complement Titration On account of the complement variability of guinea pigs' serum and of the desirability of using a uniform amount of complement, a fresh titration of the complement is done on each occasion when Wassermann tests are performed. In order to allow a margin of safety, twice that amount of guinea pigs' serum is used in the tests which in the preliminary titration gives complete laking with 2 units of amboceptor in 15 minutes. (The quantities of all the ingredients given here are known as half Wassermann quantities; that is, for the sake of economy and convenience half the amount of patients' serum, guinea pig serum, sheep cells, etc., described in the original Wassermann test are used and the total volume is made to 2.5 c.c. instead of 5 c.c. Some workers carry economy farther and use tenth Wassermann quantities. There are also some workers who instead of redetermining the dose of complement each time, keep the dose of complement constant ? 0.5 c.c. of 10 per cent serum for half Wassermanns ?. and redetermine the amboceptor unit. The net result is not very different, but since the guinea pig is the variable factor it seems more reasonable to vary the amount of guinea pigs' serum.) Into each of 7 tubes put 0.5 c.c. of 5 per cent sheep cells, two units of amboceptor and 10 per cent complement (fresh guinea pig serum) in the following amounts: Tube Sheep Cells Sensitizer Or Amboceptor Complement 10 % m Halt Solution 10.5 c.o.2 units0.1 c.c.20.5 c.c.2 units0.15 c.c.30.5 c.c.2 units0.2 c.c.40.5 c.c.2 units0.25 c.c.50.5 c.c.2 units0.3 c.c.(i0.5 c.c.2 units0.4 c.c.70.5 c.c.2 units0.5 c.c.80.5 c.c.?0.5 c.c.Bring the volume of all the tubes up to 2.5 c.c. by addition of saline solution. ...


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Product Details
  • ISBN-13: 9780217155441
  • Publisher: Rarebooksclub.com
  • Publisher Imprint: Rarebooksclub.com
  • Height: 246 mm
  • No of Pages: 30
  • Spine Width: 2 mm
  • Width: 189 mm
  • ISBN-10: 0217155448
  • Publisher Date: 09 Jul 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 73 gr


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