Techniques in Aquatic Toxicology, Volume 2

Whether considering toxicant exposure in zebrafish, or the application of cellular diagnostics to marine toxicology, or the ecotoxicology of coral reef ecosystems, or the amount of metalloids in water, this reference offers the protocols for specimen collection that researchers need. Following up on his popular Techniques in Aquatic Toxicology with

Table of Contents:
Techniques for assessment of toxicity in whole organisms Integrative measures of toxicant exposure in zebrafish (Danio rerio) at different levels of biological organization. Roel Smolders, Wim De Coen, and Ronny Blust Use of disease challenge assay to assess immunotoxicity of xenobiotics in fish. Mary R. Arkoosh, Deborah Boylen, Coral L. Stafford, Lyndal L. Johnson, and Tracy K. Collier Enhanced frog embryo teratogenesis assay - Xenopus (FETAX) model using Xenopus tropicalis.Douglas J. Fort and Robert L. Rogers A short-term mummichog (Fundulus heteroclitus) bioassay to assess endocrine responses to hormone-active compounds and mixtures. Deborah L. MacLatchy, Karen L. Gormley, Rebecca E. M. Ibey, Rainie L. Sharpe, Kevin S. Shaughnessy, Simon C. Courtenay, Monique G. Dube, and Glen J. Van Der Kraak Conducting dose-response feeding studies with salmonids: growth as an endpoint. James P. Meador, Frank C. Sommers, Robert J. Wolotira, and Leslie Kubin Field experiments with caged bivalves to assess chronic exposure and toxicity. Michael H. Salazar and Sandra M. Salazar Application of computer microscopy for histopathology in isopod toxicity studies. Damjana Drobne and Samo Drobne Sperm cell and embryo toxicity tests using the sea urchin Paracentrotus lividus (LmK). Annamaria Volpi Ghirardini, Alessandra Arizzi Novelli, Chiara Losso, and Pier Francesco Ghetti Assessment of metal toxicity to sulfate reducing bacteria through metal concentration methods. Vivek P. Utgikar, and Henry H. Tabak Techniques for measurement of cellular and subcellular toxicity Cellular diagnostics and its application to aquatic and marine toxicology. Craig A. Downs A non-destructive technique to measure cytochrome P4501A enzyme activity in living embryos of the estuarine fish Fundulus heteroclitus. Diane Nacci, Laura Coiro, Deena M. Wassenberg, and Richard T. Di Giulio Determination of lipid classes and lipid content in tissues of aquatic organisms using a thin layer chromatography/flame ionization detection (TLC/FID) microlipid method. Gina M. Ylitalo, Gladys K. Yanagida, Lawrence Hufnagle, Jr., and Margaret M. Krahn Larval molting hormone synthesis and imaginal disc development in the midge Chironomus riparius as tools for assessing the endocrine modulating potential of chemicals in aquatic insects. Torsten Hahn The electro-olfactogram: an in vivo measure of peripheral olfactory function and sublethal neurotoxicity in fish. David H. Baldwin and Nathaniel L. Scholz Enzyme-linked immunosorbent assay for screening estrogen receptor binding activity Tomoko Koda, Yoshihiro Soya, and Masatoshi Morita Lysosomal destabilization assays for estuarine organisms. A. H. Ringwood, D. E. Conners, J. Hoguet, and L. A. Ringwood IMCOMP-P: an assay for coral immuno-competence. Craig A. Downs, Aaron G. Downs, Robert B. Jonas, Kay Briggs, Thomas Capo, and Cheryl M. Woodley Monitoring gene expression in Rana catesbeiana tadpoles using a tail fin biopsy technique and its application to the detection of environmental endocrine disruptor effects in wildlife species. Nik Veldhoen and Caren C. Helbing Techniques for identification and assessment of contaminants in aquatic ecosystems Coral reproduction and recruitment as tools for studying the ecotoxicology of coral reef ecosystems. Robert H. Richmond Using the stickleback to monitor androgens and anti-androgens in the aquatic environment. Ioanna Katsiadaki Simple methods for estimating exposure concentrations of pesticide resulting from non-point source applications in agricultural drainage networks. Wenlin Chen Design and analysis of toxicity tests for the development and validation of biotic ligand models for predicting metal bioavailability and toxicity. Karel A.C. DeSchamphelaere, Dagobert G. Heijerick, and Colin R. Janssen Rapid toxicity fingerprinting of polluted waters using lux-marked bacteria. Nigel L. Turner Aquatic in situ bioassays to detect agricultural non-point source pesticide pollution: a link between laboratory and field. Ralf Schulz Improvements to high-performance liquid chromatography/photodiode array detection (HPLC/PDA) method that measures dioxin-like polychlorinated biphenyls and other selected organochlorines in marine biota. Gina M. Ylitalo, Jon Buzitis, Daryle Boyd, David P. Herman, Karen L. Tilbury, and Margaret M. Krahn Estrogenic activity measurement in wastewater using in vitro and in vivo methods. Yelena Sapozhnikova, Anne McElroy, Shane Snyder, and Daniel Schlenk A toxicity assessment approach for evaluation of in-situ bioremediation of PAH contaminated sediments. Henry H. Tabak, James M. Lazorchak, Mark E. Smith, and Jim Ferretti Application of solid-phase microextraction fibres as biomimetic sampling devices in ecotoxicology. Roman Lanno, James M. Conder, Jason B. Wells, and Thomas W. La Point Passive dosemeters for measurement of ultraviolet radiation in aquatic environments. C.S Sinclair, R.H. Richmond, E.T. Knobbe, C. Basslear, and G.K. Ostrander Techniques for aquatic toxicologists Spectral models for assessing exposure of fish to contaminants. Donald C. Malins, John J. Stegeman, Virginia M. Green, Naomi K. Gilman, and Katie M. Anderson Design and use of a highly responsive and rigidly controllable hypoxia exposure system. D. W. Lehmann, J. F. Levine, and J. M. Law Fish models in behavioral toxicology: automated techniques, updates and perspectives. Andrew S. Kane, James D. Salierno, and Sandra K. Brewer Measuring metals and metalloids in water, sediment, and biological tissues. Michael C. Newman and Yuan Zhao Estimation of inorganic species aquatic toxicity. James P. Hickey Determining aromatic hydrocarbons and chlorinated hydrocarbons in sediments and tissues using accelerated solvent extraction and gas chromatography/mass spectrometry. C.A. Sloan, D.W. Brown, R.W. Pearce, R.H. Boyer, J.L. Bolton, D.G. Burrows, D.P. Herman, and M.M. Krahn Histological preparation of invertebrates for evaluating contaminant effects. Esther C. Peters, Kathy L. Price, and Doranne J. Borsay Horowitz Isolation of genes in aquatic animals using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Jeanette M. Rotchell Analysis of mutations in ? transgenic medaka using the cll mutation assay. Richard N. Winn and Michelle B. Norris Improved methods of conducting microalgal bioassays using flow cytometry. M. Franklin, Jennifer L. Stauber, and Merrin S. Adams